ELISA Kit for Bcl2 Associated X Protein (Bax)
BCL2L4; Bax Zeta; Apoptosis regulator BAX; Bcl-2-like protein 4
- Product No.SEB343Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range62.50-4000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 26.3pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Bcl2 Associated X Protein (Bax).
No significant cross-reactivity or interference between Bcl2 Associated X Protein (Bax) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Bcl2 Associated X Protein (Bax) and the recovery rates were calculated by comparing the measured value to the expected amount of Bcl2 Associated X Protein (Bax) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2 Associated X Protein (Bax) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2 Associated X Protein (Bax) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bcl2 Associated X Protein (Bax) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|International Journal of Health Sciences||Neurotoxic effect of lead on rats: Relationship to Apoptosis NCBI: PMC3883608|
|Interactive cardiovascular and thoracic surgery||Effects of hepatocyte growth factor in myocarditis rats induced by immunization with porcine cardiac myosin Pubmed: 24327573|
|Biological Journal of Armenia||Cu (II) (PICOLINYL-L-TRYPTOPHAN)2 AS AN EFFECIENT MULTIFUNCTIONAL RADIOPROTECTOR Asj-oa: Source|
|Histochem Cell Biol.||Inhibition of small HA fragment activity and stimulation of A2A adenosine receptor pathway limit apoptosis and reduce cartilage damage in experimental arthritis Pubmed:25511416|
|Mol Cell Biochem.||The combined treatment with novel platinum(II) complex and anti-MUC1 increases apoptotic response in MDA-MB-231 breast cancer cells PubMed: 26112902|
|Histochem Cell Biol||Inhibition of small HA fragment activity and stimulation of A2A PubMed: 25511416|
|European Journal of Medicinal Plants||Hepatoprotective Effects of Rubus idaeus L. Leaves against CCl4-Induced Liver Injury via Antioxidant, Anti-inflammatory and Antiapoptotic Mechanisms openview:626440|
|Scientific Reports||In Vivo Characterization of ARN14140, a Memantine/Galantamine-Based Multi-Target Compound for Alzheimer's Disease pubmed:27609215|
|IOS Press Content Library||An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease|
|Behavioural Brain Research||Amyloid toxicity is enhanced after pharmacological or genetic invalidation of the 1 receptor pubmed:29129596|
|Biomedicine & Pharmacotherapy||Mechanistic insights into the effects of quercetin and/or GLP-1 analogue liraglutide on high-fat diet/streptozotocin-induced type 2 diabetes in rats pubmed:28554128|
|Oncology Letters||Mechanism of anticancer action of novel berenil complex of platinum(II) combined with anti-MUC1 in MCF-7 breast cancer cells 10.3892/ol.2017.7623|
|International Journal of Biological Macromolecules||A polysaccharide (PNPA) from Pleurotus nebrodensis ameliorates hepatic ischemic/reperfusion (I/R) injury in rats pubmed:28709897|
|Toxicology and Environmental Health Sciences||Potentiation of Apoptosis in Rat Striatum Exposed to Endosulfan and the Role of Quercetin 10.1007/s13530-017-0325-1|
|Biomedicine & Pharmacotherapy||Cardioprotective effect of the xanthones from Gentianella acuta against myocardial ischemia/reperfusion injury in isolated rat heart pubmed:28686977|
|Journal of Ethnopharmacology||Viscum articulatum Burm. f. aqueous extract exerts antiproliferative effect and induces cell cycle arrest and apoptosis in leukemia cells Pubmed:29555410|
|Oncology Letters||Mechanism of anticancer action of novel berenil complex of platinum (II) combined with anti-MUC1 in MCF-7 breast cancer cells Pubmed:29434943|
|Scientific Research||Association of Bax and Bcl-2 Functional Polymorphisms and Protein Levels with Posttraumatic Stress Disorder paperID:82598|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
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