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ELISA Kit for Apoptosis Signal Regulating Kinase 1 (ASK1)

MAP3-K5; MAP3K5; ASK1; MAPKKK5; MEKK5; Mitogen-Activated Protein Kinase Kinase Kinase 5; MAPK/ERK kinase kinase 5

Specificity

This assay has high sensitivity and excellent specificity for detection of Apoptosis Signal Regulating Kinase 1 (ASK1).
No significant cross-reactivity or interference between Apoptosis Signal Regulating Kinase 1 (ASK1) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apoptosis Signal Regulating Kinase 1 (ASK1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apoptosis Signal Regulating Kinase 1 (ASK1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Fish Physiology and Biochemistry Heat-shock protein 70 modulates apoptosis signal-regulating kinase 1 in stressed hepatocytes of Mugil cephalus Pubmed:24875452
Fish Physiology and Biochemistry Differential reCavia (Guinea pig )lation of pro- and antiapoptotic proteins in fish adipocytes during hypoxic conditions. Pubmed:26744268
网页打不开 ASK-1 Expression Down-regulated in Preeclamptic Placental Explants Incubated with Mint-Tea :
Mar Pollut Bull. Differential expression of survival proteins during decreased intracellular oxygen tension in brain endothelial cells of grey mullets. pubmed:28043644
 Journal of FisheriesSciences. com Upregulation of HSP70 Extends Cytoprotection to Fish Brain under Xenobiotic Stress b0407ebde042c4f61b728bc11a586e6c
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