ELISA Kit for Lactate Dehydrogenase A (LDHA)

LDH-A; LDH-M; LDH1; PIG19; Cell proliferation-inducing gene 19 protein; LDH muscle subunit; Renal carcinoma antigen NY-REN-59


This assay has high sensitivity and excellent specificity for detection of Lactate Dehydrogenase A (LDHA).
No significant cross-reactivity or interference between Lactate Dehydrogenase A (LDHA) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Lactate Dehydrogenase A (LDHA) and the recovery rates were calculated by comparing the measured value to the expected amount of Lactate Dehydrogenase A (LDHA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-98 95
EDTA plasma(n=5) 94-101 97
heparin plasma(n=5) 83-97 88


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactate Dehydrogenase A (LDHA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactate Dehydrogenase A (LDHA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lactate Dehydrogenase A (LDHA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-102% 78-97% 92-101% 78-105%
EDTA plasma(n=5) 97-105% 80-97% 84-96% 84-91%
heparin plasma(n=5) 95-104% 78-90% 82-93% 93-101%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
33 Extracellular Lactate Dehydrogenase A Release From Damaged Neurons Drives Central Nervous System Angiogenesis pubmed:29248508
International Journal of Clinical and Experimental Medicine Effects of fecal microbiota transplantation and joint application of probiotics on rats with alcoholic liver disease
Docosahexaenoic acid (DHA), a nutritional supplement, modulates steroid insensitivity in asthma
Chrysin serves as a novel inhibitor of DGKα/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC)
ACS Omega SOD1 is a Possible Predictor of COVID-19 Progression as Revealed by Plasma Proteomics 34250342
Nutrients Mokko Lactone Alleviates Doxorubicin-Induced Cardiotoxicity in Rats via Antioxidant, Anti-Inflammatory, and Antiapoptotic Activities. Nutrients 2022, 14, 733 Pubmed:35215383
Pharmaceutics Co-Delivery of Docosahexaenoic Acid and Brain-Derived Neurotropic Factor from Electrospun Aligned Core–Shell Fibrous Membranes in Treatment of Spinal Cord … Pubmed:35214053
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