ELISA Kit for Hemoglobin (HB)
Hgb; Haemoglobin; Heterotetramer(αβ)2
- Product No.SEB409Eq
- Organism SpeciesEquus caballus; Equine (Horse) Same name, Different species.
- Sample TypeSerum, plasma, erythrocyte lysates.
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range9.38-600ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 3.7ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Hemoglobin (HB).
No significant cross-reactivity or interference between Hemoglobin (HB) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Hemoglobin (HB) and the recovery rates were calculated by comparing the measured value to the expected amount of Hemoglobin (HB) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hemoglobin (HB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hemoglobin (HB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hemoglobin (HB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology||Effects of ectoine on behavioural, physiological and biochemical parameters of Daphnia magna Pubmed:25460046|
|PLoS One.||Comparative Proteomics Identifies Host Immune System Proteins Affected by Infection with Mycobacterium bovis Pubmed:27027307|
|Parasites & Vectors||The intracellular bacterium Anaplasma phagocytophilum selectively manipulates the levels of vertebrate host proteins in the tick vector Ixodes scapularis. pubmed:27561965|
|Proteomics. Clinical Applications||Elevated levels of protein AMBP in cerebrospinal fluid of women with preeclampsia compared to normotensive pregnant women. pubmed:27615121|
|Molecular nutrition & food research||Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro pubmed:28105758|
|Frontiers in Immunology||Oxidized Hemoglobin Is Antigenic and Immunogenic in Lupus pubmed:28694810|
|Catalog No.||Related products for research use of Equus caballus; Equine (Horse) Organism species||Applications (RESEARCH USE ONLY!)|
|PAB409Eq01||Polyclonal Antibody to Hemoglobin (HB)||WB; IHC; ICC; IP.|
|SEB409Eq||ELISA Kit for Hemoglobin (HB)||Enzyme-linked immunosorbent assay for Antigen Detection.|