ELISA Kit for Pancreatic Lipase (PL)

PNLIP; PTL; Pancreatic Lipase; Pancreatic triacylglycerol lipase

Specificity

This assay has high sensitivity and excellent specificity for detection of Pancreatic Lipase (PL).
No significant cross-reactivity or interference between Pancreatic Lipase (PL) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Pancreatic Lipase (PL) and the recovery rates were calculated by comparing the measured value to the expected amount of Pancreatic Lipase (PL) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-95 84
EDTA plasma(n=5) 80-91 80
heparin plasma(n=5) 78-99 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pancreatic Lipase (PL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pancreatic Lipase (PL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pancreatic Lipase (PL) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 96-105% 91-105% 97-104% 88-103%
EDTA plasma(n=5) 95-103% 97-105% 89-103% 82-97%
heparin plasma(n=5) 96-105% 80-90% 79-101% 91-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Toxicol Pathol.? Mechanistic investigations of test?article-induced?pancreatic toxicity at the endocrine-exocrine interface in the rat. Pubmed:24226507
The Korean Journal of Internal Medicine Clinical efficacy of serum lipase subtype analy-sis for the differential diagnosis of pancreatic and non-pancreatic lipase elevation Pubmed:27243230
Clinical Nutrition ESPEN Abdominal fat distribution modulates the metabolic effects of exogenous ketones in individuals with new-onset prediabetes after acute pancreatitis: Results from a randomized placebo-controlled trial 34024503
Obes Facts Associations between Intra-Pancreatic Fat Deposition, Pancreas Size, and Pancreatic Enzymes in Health and after an Attack of Acute Pancreatitis 34753126
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB453Ra01 Recombinant Pancreatic Lipase (PL) Positive Control; Immunogen; SDS-PAGE; WB.
PAB453Ra01 Polyclonal Antibody to Pancreatic Lipase (PL) WB; IHC; ICC; IP.
MAB453Ra21 Monoclonal Antibody to Pancreatic Lipase (PL) WB; IHC; ICC; IP.
SEB453Ra ELISA Kit for Pancreatic Lipase (PL) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB453Ra Multiplex Assay Kit for Pancreatic Lipase (PL) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.