ELISA Kit for Mannose Receptor C Type 1 (MRC1)

CD206; CLEC13D, CLEC13DL, MRC1L1; C-type lectin domain family 13 member D-like; Macrophage mannose receptor 1-like protein 1

Specificity

This assay has high sensitivity and excellent specificity for detection of Mannose Receptor C Type 1 (MRC1).
No significant cross-reactivity or interference between Mannose Receptor C Type 1 (MRC1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Mannose Receptor C Type 1 (MRC1) and the recovery rates were calculated by comparing the measured value to the expected amount of Mannose Receptor C Type 1 (MRC1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 93-101 97
EDTA plasma(n=5) 79-95 87
heparin plasma(n=5) 96-104 99

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose Receptor C Type 1 (MRC1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose Receptor C Type 1 (MRC1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mannose Receptor C Type 1 (MRC1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-92% 95-105% 79-92% 79-89%
EDTA plasma(n=5) 93-101% 92-101% 80-95% 94-105%
heparin plasma(n=5) 98-105% 83-95% 79-101% 84-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
PLoS One Spatiotemporal Cadence of Macrophage Polarisation in a Model of Light-Induced Retinal Degeneration PubMed: 26630454
Parasite Immunology KLF4 upregulation is involved in alternative macrophage activation during secondary Echinococcus granulosus infection Pubmed: 31407814
Inflammation Toll-Like Receptor 2–Mediated Autophagy Promotes Microglial Cell Death by Modulating the Microglial M1/M2 Phenotype Pubmed: 31834572
International Journal of Clinical and Experimental Medicine The phenotypical induction and damage effect of peptidoglycan on microglia
Neurochem Res Protective Effect of Mild Hypothermia on Spinal Cord Ischemia-Induced Delayed Paralysis and Spinal Cord Injury Pubmed:34993705
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB542Mu01 Recombinant Mannose Receptor C Type 1 (MRC1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB542Mu02 Recombinant Mannose Receptor C Type 1 (MRC1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB542Mu01 Polyclonal Antibody to Mannose Receptor C Type 1 (MRC1) WB; IHC; ICC; IP.
PAB542Mu02 Polyclonal Antibody to Mannose Receptor C Type 1 (MRC1) WB; IHC; ICC; IP.
SEB542Mu ELISA Kit for Mannose Receptor C Type 1 (MRC1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB542Mu Multiplex Assay Kit for Mannose Receptor C Type 1 (MRC1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.