ELISA Kit for Golgi Phosphoprotein 2 (GOLPH2)

GOLM1; GP73; C9orf155; Golgi Membrane Protein 1; Golgi Protein 73

Specificity

This assay has high sensitivity and excellent specificity for detection of Golgi Phosphoprotein 2 (GOLPH2).
No significant cross-reactivity or interference between Golgi Phosphoprotein 2 (GOLPH2) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Golgi Phosphoprotein 2 (GOLPH2) and the recovery rates were calculated by comparing the measured value to the expected amount of Golgi Phosphoprotein 2 (GOLPH2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-104 94
EDTA plasma(n=5) 94-101 97
heparin plasma(n=5) 80-90 87

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Golgi Phosphoprotein 2 (GOLPH2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Golgi Phosphoprotein 2 (GOLPH2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Golgi Phosphoprotein 2 (GOLPH2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-101% 89-99% 78-97% 78-103%
EDTA plasma(n=5) 86-94% 96-105% 89-103% 93-101%
heparin plasma(n=5) 98-105% 91-101% 78-91% 95-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Molecular & Cellular Proteomics LAMC2: A promising new pancreatic cancer biomarker identified by proteomic analysis of pancreatic adenocarcinoma tissues Mcponline: 023507
Afro-Egypt J Infect Endem Dis Diagnostic and Prognostic Validity of Serum Golgi Protein 73 in Egyptian Patients with Hepatocelluar Carcinoma Ajied: Ajied_System_Files
Journal of Applied Pharmaceutical Science Diagnostic role of Golgi protein 73 and IL-17 in Egyptian cirrhotic rather than hepatocellular carcinoma patients. handle:123456789
Oncology Letters Serum Golgi protein 73 is a prognostic rather than diagnostic marker in hepatocellular carcinoma pubmed:29113278
Scientific Reports The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study Pubmed: 32198440
Hepatobiliary & Pancreatic Diseases International Dynamic expression of hepatic GP73 mRNA and protein and circulating GP73 during hepatocytes malignant transformation Pubmed: 32171652
Biomolecules Deoxycholic Acid Upregulates Serum Golgi Protein 73 through Activating NF-¦ÊB Pathway and Destroying Golgi Structure in Liver Disease. Biomolecules 2021, 11?¡­ 33540642
ADVANCED RESEARCHES Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C
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