ELISA Kit for Cluster Of Differentiation 146 (CD146)

MCAM; MUC18; Melanoma Cell Adhesion Molecule; Cell surface glycoprotein P1H12; Melanoma-associated antigen A32; S-endo 1 endothelial-associated antigen

Specificity

This assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 146 (CD146).
No significant cross-reactivity or interference between Cluster Of Differentiation 146 (CD146) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Cluster Of Differentiation 146 (CD146) and the recovery rates were calculated by comparing the measured value to the expected amount of Cluster Of Differentiation 146 (CD146) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-96 90
EDTA plasma(n=5) 78-97 82
heparin plasma(n=5) 95-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 146 (CD146) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 146 (CD146) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cluster Of Differentiation 146 (CD146) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-101% 82-89% 78-95% 87-101%
EDTA plasma(n=5) 81-94% 82-98% 91-102% 78-101%
heparin plasma(n=5) 78-89% 95-103% 81-91% 93-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Clinical Rheumatology Evaluation of various endothelial biomarkers in ankylosing spondylitis PubMed: 21556780
Inflammation The Effect of Regular Colchicine Treatment on Biomarkers Related with Vascular Injury in Newly Diagnosed Patients with Familial Mediterranean Fever SpringerLink: m7128503g576w808
Oncotarget Selective targeting of liver cancer with the endothelial marker CD146 Pubmed:25238265
Future Medicine Melanoma cell adhesion molecule as an emerging biomarker with prognostic significance in systolic heart failure pubmed:27355366
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB670Mu02 Recombinant Cluster Of Differentiation 146 (CD146) Positive Control; Immunogen; SDS-PAGE; WB.
RPB670Mu01 Recombinant Cluster Of Differentiation 146 (CD146) Positive Control; Immunogen; SDS-PAGE; WB.
PAB670Mu02 Polyclonal Antibody to Cluster Of Differentiation 146 (CD146) WB; IHC; ICC; IP.
PAB670Mu01 Polyclonal Antibody to Cluster Of Differentiation 146 (CD146) WB; IHC; ICC; IP.
LAB670Mu81 FITC-Linked Polyclonal Antibody to Cluster Of Differentiation 146 (CD146) WB; IHC; ICC; IF.
LAB670Mu71 Biotin-Linked Polyclonal Antibody to Cluster Of Differentiation 146 (CD146) WB; IHC; ICC.
SEB670Mu ELISA Kit for Cluster Of Differentiation 146 (CD146) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB670Mu CLIA Kit for Cluster Of Differentiation 146 (CD146) Chemiluminescent immunoassay for Antigen Detection.
LMB670Mu Multiplex Assay Kit for Cluster Of Differentiation 146 (CD146) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.