ELISA Kit for Annexin A2 (ANXA2)

ANX-A2; ANX2L4; CAL1H; LIP2; LPC2; LPC2D; P36; PAP-IV; Protein I; Chromobindin-8; Calpactin-1 heavy chain; Lipocortin II; Placental anticoagulant protein IV

Specificity

This assay has high sensitivity and excellent specificity for detection of Annexin A2 (ANXA2).
No significant cross-reactivity or interference between Annexin A2 (ANXA2) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Annexin A2 (ANXA2) and the recovery rates were calculated by comparing the measured value to the expected amount of Annexin A2 (ANXA2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-102 95
EDTA plasma(n=5) 79-102 95
heparin plasma(n=5) 81-94 85

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A2 (ANXA2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A2 (ANXA2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Annexin A2 (ANXA2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-97% 86-93% 81-91% 89-101%
EDTA plasma(n=5) 95-105% 88-98% 80-99% 92-99%
heparin plasma(n=5) 84-98% 95-105% 95-103% 91-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Journal of Cellular Biochemistry Subcellular proteome analysis unraveled annexin A2 related to immune liver fibrosis PubMed: 20225235
World Journal of Gastroenterology Expression characteristics and diagnostic value of annexin A2 in hepatocellular carcinoma PubMed: PMC3491596
Oncology Letters Annexin A2 is not a good biomarker for hepatocellular carcinoma in cirrhosis PubMed: PMC3742823
Ibrahim Annexin A2 Versus Afp as An Efficient Diagnostic Serum Marker for Hepatocellular Carcinoma Ghrnet: Source
European Journal of Clinical Microbiology &amp; Infectious Diseases Serum annexin A2 levels in acute brucellosis and brucellar spondylodiscitis Pubmed:24853056
Biomedicine &amp; Pharmacotherapy Circulating annexin A2 as a biomarker in gastric cancer patients: Correlation with clinical variables Pubmed:25661364
Hepat Mon Serum Levels of Annexin A2 as a Candidate Biomarker for Hepatic Fibrosis in Patients With Chronic Hepatitis B PubMed: 26587036
Tumour Biol Up-regulation of annexin A2 expression predicates advanced clinicopathological features and poor prognosis in hepatocellular carcinoma PubMed: 26109000
Biomed Pharmacother Circulating PubMed: 25661364
Tumour Biol Evaluation of annexin A2 and as potential biomarkers for hepatocellular carcinoma PubMed: 26189841
Eur J Clin Invest Stairs instead of elevators at the workplace decreases PCSK9 levels in a healthy population PubMed: 26081791
Journal of Oral and Maxillofacial Surgery Serum Annexin A2 Level Is Associated With Diagnosis and Prognosis in Patients With OralSquamous Cell Carcinoma. pubmed:27889534
Pregnancy Hypertens Assessment of annexin A5 and annexin A2 levels as biomarkers for pre-eclampsia: A pilot study. pubmed:28501283
Mol Nutr Food Res. Epigallocatechin gallate induces an up-regulation of LDL receptor accompanied by a reduction of PCSK9 via the annexin A2-independent pathway in HepG2 cells. pubmed:28181408
Archives of Gynecology and Obstetrics Expression of annexin A2 in adenomyosis and dysmenorrhea Pubmed: 31183557
Cancers Extracellular Vesicles-Based Biomarkers Represent a Promising Liquid Biopsy in Endometrial Cancer Pubmed: 31842290
PLASMA BIOMARKER FOR OVARIAN CANCER
Diagnostics Diagnostic Value of Plasma Annexin A2 in Early-Stage High-Grade Serous Ovarian Cancer. Diagnostics 2021, 11, 69 33406648
ADVANCED RESEARCHES Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C
Cancer Cell International Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins
Cancer Cell Int Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression 34376212
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