ELISA Kit for Tenascin C (TNC)

TN-C; HXB; TN; GMEM; JI; Myotendinous antigen; Neuronectin; GP 150-225; Cytotactin; Hexabrachion; Glioma-associated-extracellular matrix antigen

Specificity

This assay has high sensitivity and excellent specificity for detection of Tenascin C (TNC).
No significant cross-reactivity or interference between Tenascin C (TNC) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Tenascin C (TNC) and the recovery rates were calculated by comparing the measured value to the expected amount of Tenascin C (TNC) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-98 94
EDTA plasma(n=5) 80-102 86
heparin plasma(n=5) 96-103 99

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tenascin C (TNC) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tenascin C (TNC) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tenascin C (TNC) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-101% 83-102% 88-95% 90-102%
EDTA plasma(n=5) 87-103% 95-103% 92-99% 93-102%
heparin plasma(n=5) 82-93% 94-103% 89-103% 78-90%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Medical Principles and Practice An investigation of tenascin-C levels in rheumatic mitral stenosis and their response to percutaneous mitral balloon valvuloplasty Pubmed: 22889719
Tumor Biology Clinical significance of serum tenascin-c levels in epithelial ovarian cancer Springer: Source
Tumor Biology Clinical significance of serum tenascin-C levels in breast cancer Springer: Source
BioFactors Tenascin-C is increased in atherothrombotic stroke patients and has an anti-inflammatory effect in the human carotid artery. Pubmed:24823872
Connective Tissue Research Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes Pubmed:25333747
K Role of Tenascin-C in the differential diagnosis of small round blue cell tumors Index.Php: Sdutfd
Life Sci A new predictor of mortality in hemodialysis patients; Tenascin-C PubMed: 26390818
Heart Research Biomarkers Score for Patients with Mitral Stenosis: A useful conjunction with Wilkins’s Score for Early Intervention Heartresearchopenjournal: Hroj5
Araştırmalar / Researches Pankreas Adenokarsinomunda Serum Tenascin-C Düzeyi Potansiyel Bir Biyobelirteç Midir? Cpdf:5C21620161255
Archives of Dermatological Research Increased tenascin C and DKK1 in vitiligo: possible role of fibroblasts in acral and non-acral disease Pubmed:29605863
Methods in Cell Biology How to detect and purify tenascin-C Pubmed:29310788
Scientific Reports Altered transcriptional regulatory proteins in glioblastoma and YBX1 as a potential regulator of tumor invasion Pubmed: 31358880
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB975Hu02 Recombinant Tenascin C (TNC) Positive Control; Immunogen; SDS-PAGE; WB.
RPB975Hu01 Recombinant Tenascin C (TNC) Positive Control; Immunogen; SDS-PAGE; WB.
PAB975Hu01 Polyclonal Antibody to Tenascin C (TNC) WB; IHC; ICC; IP.
PAB975Hu02 Polyclonal Antibody to Tenascin C (TNC) WB; IHC; ICC; IP.
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MAB975Hu22 Monoclonal Antibody to Tenascin C (TNC) WB; IHC; ICC; IP.
MAB975Hu21 Monoclonal Antibody to Tenascin C (TNC) WB; IHC; ICC; IP.
MAB975Hu23 Monoclonal Antibody to Tenascin C (TNC) WB; IHC; ICC; IP.
MAB975Hu24 Monoclonal Antibody to Tenascin C (TNC) WB; IHC; ICC; IP.
SEB975Hu ELISA Kit for Tenascin C (TNC) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB975Hu Multiplex Assay Kit for Tenascin C (TNC) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.