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ELISA Kit for Survivin (Surv)

BIRC5; API4; EPR-1; Baculoviral Inhibitor Of Apoptosis Repeat Containing 5; Apoptosis Inhibitor 4; Survivin Variant 3 Alpha

Specificity

This assay has high sensitivity and excellent specificity for detection of Survivin (Surv).
No significant cross-reactivity or interference between Survivin (Surv) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Survivin (Surv) and the recovery rates were calculated by comparing the measured value to the expected amount of Survivin (Surv) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-93 88
EDTA plasma(n=5) 88-101 97
heparin plasma(n=5) 92-99 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Survivin (Surv) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Survivin (Surv) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Survivin (Surv) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-90% 83-91% 80-94% 93-105%
EDTA plasma(n=5) 98-105% 90-101% 90-97% 95-102%
heparin plasma(n=5) 82-96% 84-92% 83-103% 92-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Reference
Prion. Heligmosomoides polygyrus antigens inhibit the intrinsic pathway of apoptosis by overexpression of survivin and Bcl-2 protein in CD4 T cells Pubmed: 23787700
Medical Oncology Expression of serum survivin protein in diagnosis and prognosis of gallbladder cancer: a comparative study Pubmed:25129311
J Surg Res. Prognostic significance of survivin in resected gallbladder cancer Pubmed:25472573
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC045Hu01 Recombinant Survivin (Surv) Positive Control; Immunogen; SDS-PAGE; WB.
PAC045Hu01 Polyclonal Antibody to Survivin (Surv) WB; IHC; ICC; IP.
LAC045Hu81 FITC-Linked Polyclonal Antibody to Survivin (Surv) WB; IHC; ICC; IF.
LAC045Hu71 Biotin-Linked Polyclonal Antibody to Survivin (Surv) WB; IHC; ICC.
MAC045Hu22 Monoclonal Antibody to Survivin (Surv) WB; IHC; ICC; IP.
LAC045Hu82 FITC-Linked Monoclonal Antibody to Survivin (Surv) WB; IHC; ICC; IF.
LAC045Hu72 Biotin-Linked Monoclonal Antibody to Survivin (Surv) WB; IHC; ICC.
SEC045Hu ELISA Kit for Survivin (Surv) Enzyme-linked immunosorbent assay for Antigen Detection.
KSC045Hu01 ELISA Kit DIY Materials for Survivin (Surv) Main materials for "Do It (ELISA Kit) Yourself".