ELISA Kit for Receptor Activator Of Nuclear Factor Kappa B (RANk)

CD265; TNFRSF11A; TNFRSF11-A; ODFR; OFE; TRANCER; Osteoclast differentiation factor receptor; Tumor Necrosis Factor Receptor Superfamily,Member 11a,NFKB Activator

Specificity

This assay has high sensitivity and excellent specificity for detection of Receptor Activator Of Nuclear Factor Kappa B (RANk).
No significant cross-reactivity or interference between Receptor Activator Of Nuclear Factor Kappa B (RANk) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Receptor Activator Of Nuclear Factor Kappa B (RANk) and the recovery rates were calculated by comparing the measured value to the expected amount of Receptor Activator Of Nuclear Factor Kappa B (RANk) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-105 98
EDTA plasma(n=5) 83-95 87
heparin plasma(n=5) 85-101 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor Activator Of Nuclear Factor Kappa B (RANk) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor Activator Of Nuclear Factor Kappa B (RANk) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Receptor Activator Of Nuclear Factor Kappa B (RANk) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-91% 85-97% 80-102% 78-90%
EDTA plasma(n=5) 89-104% 89-105% 82-104% 88-102%
heparin plasma(n=5) 94-102% 79-93% 92-102% 81-88%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
European Journal of Immunology Human polymorphonuclear neutrophils express the receptor activator of NFκB (RANK) and are activated by its ligand RANKL Wiley: source
International Journal of Cardiology Valvular osteoclasts in calcification and aortic valve stenosis severity PubMed: 23452891
Bulletin  of  Experimental  Biology  and  Medicine Receptor Activator of Nuclear Transcription Factor NF-κB(RANK), Its Ligand RANKL, and Natural Inhibitor of RANKL Osteoprotegerin (OPG) in the Blood Serum of Patients with Primary Bone Tumors DOI:110.1007/s10517-017-3832-9
BIOSCIENCE REPORTS IL-38 restrains inflammatory response of collagen-induced arthritis in rats via SIRT1/HIF-1α signaling pathway Pubmed: 32347300
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPC057Ra01 Recombinant Receptor Activator Of Nuclear Factor Kappa B (RANk) Positive Control; Immunogen; SDS-PAGE; WB.
PAC057Ra01 Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B (RANk) WB; IHC; ICC; IP.
MAC057Ra21 Monoclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B (RANk) WB; IHC; ICC; IP.
SEC057Ra ELISA Kit for Receptor Activator Of Nuclear Factor Kappa B (RANk) Enzyme-linked immunosorbent assay for Antigen Detection.