ELISA Kit for E1A Binding Protein P300 (EP300)

KAT3B; p300; Histone Acetyltransferase p300

Specificity

This assay has high sensitivity and excellent specificity for detection of E1A Binding Protein P300 (EP300).
No significant cross-reactivity or interference between E1A Binding Protein P300 (EP300) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level E1A Binding Protein P300 (EP300) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level E1A Binding Protein P300 (EP300) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
BMJ Open Diabetes Research & Care miR-200b Mediates Endothelial-to-Mesenchymal Transition in Diabetic Cardiomyopathy Pubmed:26718496
Nature Communications Reducing histone acetylation rescues cognitive deficits in a mouse model of Fragile X syndrome Pubmed:29950602
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC216Mu01 Recombinant E1A Binding Protein P300 (EP300) Positive Control; Immunogen; SDS-PAGE; WB.
RPC216Mu02 Recombinant E1A Binding Protein P300 (EP300) Positive Control; Immunogen; SDS-PAGE; WB.
PAC216Mu01 Polyclonal Antibody to E1A Binding Protein P300 (EP300) WB; IHC; ICC; IP.
PAC216Mu02 Polyclonal Antibody to E1A Binding Protein P300 (EP300) WB; IHC; ICC; IP.
SEC216Mu ELISA Kit for E1A Binding Protein P300 (EP300) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC216Mu Multiplex Assay Kit for E1A Binding Protein P300 (EP300) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.