ELISA Kit for Platelet Derived Growth Factor Subunit B (PDGFB)

PDGF-B; PDGF2; SIS; SSV; C-sis; Simian Sarcoma Viral(v-sis)Oncogene Homolog; Platelet Derived Growth Factor Beta Polypeptide; Proto-oncogene c-Sis; Becaplermin

Specificity

This assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor Subunit B (PDGFB).
No significant cross-reactivity or interference between Platelet Derived Growth Factor Subunit B (PDGFB) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Platelet Derived Growth Factor Subunit B (PDGFB) and the recovery rates were calculated by comparing the measured value to the expected amount of Platelet Derived Growth Factor Subunit B (PDGFB) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-92 87
EDTA plasma(n=5) 82-105 98
heparin plasma(n=5) 82-98 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit B (PDGFB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit B (PDGFB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Platelet Derived Growth Factor Subunit B (PDGFB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-101% 81-98% 84-97% 78-105%
EDTA plasma(n=5) 96-104% 79-89% 81-95% 78-92%
heparin plasma(n=5) 80-103% 90-97% 85-98% 82-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
AAPS PharmSciTech Inhibition of Glomerular Mesangial Cell Proliferation by siPDGF-B-and siPDGFR-β-Containing Chitosan Nanoplexes pubmed:27975193
Marmara Pharmaceutical Journal In vitro PDGF-B gene silencing studies and In vivo delivery of siRNA to the rat kidney using Chitosan/siRNA nanoplexes 5000184338
International Journal of Clinical and Experimental Pathology Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens ISSN:1936-2625/IJCEP0058424
Cell Biology International Melatonin promotes osteoblastic differentiation and regulates PDGF/AKT signaling pathway Pubmed: 31535749
Ann Agric Environ Med PDGF-BB homodimer serum level–a good indicator of the severity of alcoholic liver cirrhosis Pubmed: 32208584
Journal of Tissue Engineering Therapeutic potential of adipose-derived mesenchymal stem cell exosomes in tissue-engineered bladders 33868627
Catalog No. Related products for research use of Sus scrofa; Porcine (Pig) Organism species Applications (RESEARCH USE ONLY!)
RPC921Po01 Recombinant Platelet Derived Growth Factor Subunit B (PDGFB) Positive Control; Immunogen; SDS-PAGE; WB.
PAC921Po01 Polyclonal Antibody to Platelet Derived Growth Factor Subunit B (PDGFB) WB; IHC; ICC; IP.
MAC921Po21 Monoclonal Antibody to Platelet Derived Growth Factor Subunit B (PDGFB) WB; IHC; ICC; IP.
SEC921Po ELISA Kit for Platelet Derived Growth Factor Subunit B (PDGFB) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC921Po CLIA Kit for Platelet Derived Growth Factor Subunit B (PDGFB) Chemiluminescent immunoassay for Antigen Detection.
LMC921Po Multiplex Assay Kit for Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.