ELISA Kit for Ferritin, Heavy Polypeptide (FTH)
FTH1; FTHL6; PIG15; PLIF; Ferritin Heavy Chain; Apoferritin; Placenta Immunoregulatory Factor; Proliferation-Inducing Protein 15; Cell proliferation-inducing gene 15 protein
- Product No.SED021Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range3.12-200ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.29ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Ferritin, Heavy Polypeptide (FTH).
No significant cross-reactivity or interference between Ferritin, Heavy Polypeptide (FTH) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Ferritin, Heavy Polypeptide (FTH) and the recovery rates were calculated by comparing the measured value to the expected amount of Ferritin, Heavy Polypeptide (FTH) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ferritin, Heavy Polypeptide (FTH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ferritin, Heavy Polypeptide (FTH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ferritin, Heavy Polypeptide (FTH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|PLoS One||Presence in the Pre-Surgical Fine-Needle Aspiration of Potential Thyroid Biomarkers Previously Identified in the Post-Surgical One PubMed: PMC3765839|
|Am J Physiol Lung Cell Mol Physiol.||Methemoglobin-induced signaling and chemokine responses in human alveolar epithelial cells Pubmed:24142518|
|J Alzheimers Dis||The Oral Iron Chelator, Deferasirox, Reverses the Age-Dependent Alterations in Iron and Amyloid-β Homeostasis in Rat Brain: Implications in the Therapy of Alzheimer’s Disease PubMed: 26484920|
|Eur Radiol||MR molecular imaging of tumours using ferritin heavy chain reporter gene expression mediated by the hTERT promoter Pubmed:26960542|
|Exp Hematol.||Impact of TET2 deficiency on iron metabolism in erythroblasts. pubmed:28167288|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|RPD021Ra01||Recombinant Ferritin, Heavy Polypeptide (FTH)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAD021Ra01||Polyclonal Antibody to Ferritin, Heavy Polypeptide (FTH)||WB; IHC; ICC; IP.|
|LAD021Ra71||Biotin-Linked Polyclonal Antibody to Ferritin, Heavy Polypeptide (FTH)||WB; IHC; ICC.|
|MAD021Ra21||Monoclonal Antibody to Ferritin, Heavy Polypeptide (FTH)||WB; IHC; ICC; IP.|
|SED021Ra||ELISA Kit for Ferritin, Heavy Polypeptide (FTH)||Enzyme-linked immunosorbent assay for Antigen Detection.|