ELISA Kit for Acid Phosphatase 2, Lysosomal (ACP2)

Lysosomal Acid Phosphatase

Specificity

This assay has high sensitivity and excellent specificity for detection of Acid Phosphatase 2, Lysosomal (ACP2).
No significant cross-reactivity or interference between Acid Phosphatase 2, Lysosomal (ACP2) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Acid Phosphatase 2, Lysosomal (ACP2) and the recovery rates were calculated by comparing the measured value to the expected amount of Acid Phosphatase 2, Lysosomal (ACP2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-95 91
EDTA plasma(n=5) 92-99 95
heparin plasma(n=5) 96-104 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acid Phosphatase 2, Lysosomal (ACP2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acid Phosphatase 2, Lysosomal (ACP2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Acid Phosphatase 2, Lysosomal (ACP2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-101% 91-98% 98-105% 78-90%
EDTA plasma(n=5) 81-93% 93-101% 97-105% 79-89%
heparin plasma(n=5) 89-104% 97-105% 80-101% 91-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Diabetes Influences the Fusion of Autophagosomes with Lysosomes in SH-SY5Y Cells and Induces Aβ Deposition and Cognitive Dysfunction in STZ-Induced Diabetic …
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPD089Hu01 Recombinant Acid Phosphatase 2, Lysosomal (ACP2) Positive Control; Immunogen; SDS-PAGE; WB.
PAD089Hu01 Polyclonal Antibody to Acid Phosphatase 2, Lysosomal (ACP2) WB
MAD089Hu21 Monoclonal Antibody to Acid Phosphatase 2, Lysosomal (ACP2) WB; IHC; ICC; IP.
SED089Hu ELISA Kit for Acid Phosphatase 2, Lysosomal (ACP2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD089Hu Multiplex Assay Kit for Acid Phosphatase 2, Lysosomal (ACP2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.