ELISA Kit for Cyclin B (CCNB)

CCNB1; CCN-B; G2/Mitotic-Specific Cyclin B1

Specificity

This assay has high sensitivity and excellent specificity for detection of Cyclin B (CCNB).
No significant cross-reactivity or interference between Cyclin B (CCNB) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclin B (CCNB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclin B (CCNB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Medicine & Healthcare  Evaluation of the Secretome Profile and Functional Characteristics of Human Bone Marrow Mesenchymal Stromal Cells-Derived Conditioned Medium Suggest Potential for Skin Rejuvenation 74819
Journal of Cosmetics, Dermatological Sciences and Applications Evaluation of the Secretome Profile and Functional Characteristics of Human Bone Marrow Mesenchymal Stromal Cells-Derived Conditioned Medium Suggest Potential for Skin Rejuvenation DOI: 10.4236/jcdsa.2017.71010
The effect of vitrification after warming on the expressions of p38, CDK1, and cyclin B in immature goat oocytes followed by in vitro maturation
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPD264Hu01 Recombinant Cyclin B (CCNB) Positive Control; Immunogen; SDS-PAGE; WB.
PAD264Hu01 Polyclonal Antibody to Cyclin B (CCNB) WB
MAD264Hu21 Monoclonal Antibody to Cyclin B (CCNB) WB; IHC; ICC; IP.
SED264Hu ELISA Kit for Cyclin B (CCNB) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD264Hu Multiplex Assay Kit for Cyclin B (CCNB) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.