ELISA Kit for Receptor Interacting Serine Threonine Kinase 1 (RIPK1)

RIP; Receptor-Interacting Protein; Cell death protein RIP; Serine/threonine-protein kinase RIP

Specificity

This assay has high sensitivity and excellent specificity for detection of Receptor Interacting Serine Threonine Kinase 1 (RIPK1).
No significant cross-reactivity or interference between Receptor Interacting Serine Threonine Kinase 1 (RIPK1) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor Interacting Serine Threonine Kinase 1 (RIPK1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor Interacting Serine Threonine Kinase 1 (RIPK1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Atherosclerosis Vascular peroxide 1 promotes ox-LDL-induced programmed necrosis in endothelial cells through a mechanism involving β-catenin signaling Pubmed:29772481
Cell and Tissue Research Isolation and characterization of cluster of differentiation 9-positive ependymal cells as potential adult neural stem/progenitor cells in the third ventricle of adult rats Pubmed: 31788760
Am J Respir Cell Mol Biol Persistently Elevated Plasma Levels of RIPK3, MLKL, HMGB1, and RIPK1 in COVID-19 ICU Patients Pubmed:35385375
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPE640Ra01 Recombinant Receptor Interacting Serine Threonine Kinase 1 (RIPK1) Positive Control; Immunogen; SDS-PAGE; WB.
PAE640Ra01 Polyclonal Antibody to Receptor Interacting Serine Threonine Kinase 1 (RIPK1) WB; IHC; ICC; IP.
MAE640Ra21 Monoclonal Antibody to Receptor Interacting Serine Threonine Kinase 1 (RIPK1) WB; IHC; ICC; IP.
SEE640Ra ELISA Kit for Receptor Interacting Serine Threonine Kinase 1 (RIPK1) Enzyme-linked immunosorbent assay for Antigen Detection.
LME640Ra Multiplex Assay Kit for Receptor Interacting Serine Threonine Kinase 1 (RIPK1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.