ELISA Kit for Annexin A4 (ANXA4)

ANX-A4; ANX4; PIG28; 35-beta calcimedin; Carbohydrate-binding protein p33/p41; Chromobindin-4; Endonexin I; Lipocortin IV; Placental anticoagulant protein II

Specificity

This assay has high sensitivity and excellent specificity for detection of Annexin A4 (ANXA4).
No significant cross-reactivity or interference between Annexin A4 (ANXA4) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A4 (ANXA4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A4 (ANXA4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPE784Hu01 Recombinant Annexin A4 (ANXA4) Positive Control; Immunogen; SDS-PAGE; WB.
RPE784Hu02 Recombinant Annexin A4 (ANXA4) Positive Control; Immunogen; SDS-PAGE; WB.
RPE784Hu03 Recombinant Annexin A4 (ANXA4) Positive Control; Immunogen; SDS-PAGE; WB.
PAE784Hu01 Polyclonal Antibody to Annexin A4 (ANXA4) WB; IHC; ICC; IP.
PAE784Hu02 Polyclonal Antibody to Annexin A4 (ANXA4) WB; IHC; ICC; IP.
FAE784Hu01 Anti-Annexin A4 (ANXA4) Polyclonal Antibody Flow cytometry.
LAE784Hu71 Biotin-Linked Polyclonal Antibody to Annexin A4 (ANXA4) WB; IHC; ICC.
MAE784Hu24 Monoclonal Antibody to Annexin A4 (ANXA4) WB; IHC; ICC; IP.
MAE784Hu22 Monoclonal Antibody to Annexin A4 (ANXA4) WB; IHC; ICC; IP.
MAE784Hu23 Monoclonal Antibody to Annexin A4 (ANXA4) WB
MAE784Hu21 Monoclonal Antibody to Annexin A4 (ANXA4) WB; IHC; ICC; IP.
MAE784Hu25 Monoclonal Antibody to Annexin A4 (ANXA4) WB; IHC; ICC; IP.
MAE784Hu27 Monoclonal Antibody to Annexin A4 (ANXA4) WB; IHC; ICC; IP.
MAE784Hu29 Monoclonal Antibody to Annexin A4 (ANXA4) WB; IHC; ICC; IP.
SEE784Hu ELISA Kit for Annexin A4 (ANXA4) Enzyme-linked immunosorbent assay for Antigen Detection.
LME784Hu Multiplex Assay Kit for Annexin A4 (ANXA4) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.