ELISA Kit for Lysyl Oxidase Like Protein 2 (LOXL2)

LOR2; WS9-14; Lysyl oxidase homolog 2; Lysyl oxidase-related protein 2; Lysyl oxidase-related protein WS9-14

Specificity

This assay has high sensitivity and excellent specificity for detection of Lysyl Oxidase Like Protein 2 (LOXL2).
No significant cross-reactivity or interference between Lysyl Oxidase Like Protein 2 (LOXL2) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Lysyl Oxidase Like Protein 2 (LOXL2) and the recovery rates were calculated by comparing the measured value to the expected amount of Lysyl Oxidase Like Protein 2 (LOXL2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-98 95
EDTA plasma(n=5) 98-105 101
heparin plasma(n=5) 88-95 91

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysyl Oxidase Like Protein 2 (LOXL2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysyl Oxidase Like Protein 2 (LOXL2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lysyl Oxidase Like Protein 2 (LOXL2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-101% 88-101% 88-103% 79-99%
EDTA plasma(n=5) 79-94% 85-99% 81-94% 78-103%
heparin plasma(n=5) 98-105% 87-96% 78-98% 83-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
BMC Cancer. Validation of four candidate pancreatic cancer serological biomarkers that improve the performance of CA19.12 Pubmed: 24007603
Current Eye Research High Glucose Induced Differential Expression of Lysyl Oxidase and Its Isoform in ARPE-19 Cells Pubmed: 22966818
Curr Eye Res Correlation of Aqueous Humor Lysyl Oxidase Activity with TGF-ß Levels and LOXL1 Genotype in Pseudoexfoliation Pubmed:27116380
BMC Cancer Novel biomarker-based model for the prediction of sorafenib response and overall survival in advanced hepatocellular carcinoma: a prospective cohort study Pubmed:29558905
Laryngoscope Elevated exosomal lysyl oxidase like 2 is a potential biomarker for head and neck squamous cell carcinoma Pubmed: 31219623
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