ELISA Kit for AF4/FMR2 Family, Member 1 (AFF1)

AF-4; AF4; AF4-MLL; MLL/AF4; MLLT2; PBM1; Myeloid/Lymphoid Or Mixed-Lineage Leukemia; Translocated To 2; Pre-B-Cell Monocytic Leukemia Partner 1

Specificity

This assay has high sensitivity and excellent specificity for detection of AF4/FMR2 Family, Member 1 (AFF1).
No significant cross-reactivity or interference between AF4/FMR2 Family, Member 1 (AFF1) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AF4/FMR2 Family, Member 1 (AFF1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AF4/FMR2 Family, Member 1 (AFF1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Am J Blood Res AF4 and AF4N protein complexes: recruitment of P-TEFb kinase, their interactome and potential functions PubMed: 26171280
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPJ872Hu01 Recombinant AF4/FMR2 Family, Member 1 (AFF1) Positive Control; Immunogen; SDS-PAGE; WB.
PAJ872Hu01 Polyclonal Antibody to AF4/FMR2 Family, Member 1 (AFF1) WB; IHC; ICC; IP.
MAJ872Hu21 Monoclonal Antibody to AF4/FMR2 Family, Member 1 (AFF1) WB; IHC; ICC; IP.
SEJ872Hu ELISA Kit for AF4/FMR2 Family, Member 1 (AFF1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMJ872Hu Multiplex Assay Kit for AF4/FMR2 Family, Member 1 (AFF1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.