≡

ELISA Kit for Basic Helix Loop Helix Domain Containing Protein B3 (BHLHB3)

DEC2; SHARP1; bHLHe41; Class E Basic Helix Loop Helix Protein 41; Differentially expressed in chondrocytes protein 2; Enhancer-of-split and hairy-related protein 1

Specificity

This assay has high sensitivity and excellent specificity for detection of Basic Helix Loop Helix Domain Containing Protein B3 (BHLHB3).
No significant cross-reactivity or interference between Basic Helix Loop Helix Domain Containing Protein B3 (BHLHB3) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Basic Helix Loop Helix Domain Containing Protein B3 (BHLHB3) and the recovery rates were calculated by comparing the measured value to the expected amount of Basic Helix Loop Helix Domain Containing Protein B3 (BHLHB3) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 94-101 98
EDTA plasma(n=5) 86-94 91
heparin plasma(n=5) 97-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Basic Helix Loop Helix Domain Containing Protein B3 (BHLHB3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Basic Helix Loop Helix Domain Containing Protein B3 (BHLHB3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Basic Helix Loop Helix Domain Containing Protein B3 (BHLHB3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-94% 83-101% 83-91% 84-93%
EDTA plasma(n=5) 97-104% 80-96% 99-105% 78-96%
heparin plasma(n=5) 79-91% 95-103% 92-104% 80-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Scientific Reports Serum SHARP1 and uterine artery Doppler for the prediction of preeclampsia Pubmed: 31439869
Journal of Maternal-Fetal & Neonatal Medicine Maternal serum split and hairy-related protein 1 in preeclampsia versus normal pregnancy Pubmed: 33207988
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
SEL257Hu ELISA Kit for Basic Helix Loop Helix Domain Containing Protein B3 (BHLHB3) Enzyme-linked immunosorbent assay for Antigen Detection.
LML257Hu Multiplex Assay Kit for Basic Helix Loop Helix Domain Containing Protein B3 (BHLHB3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.