ELISA Kit for Keratin 81 (KRT81)

KRTHB1; Hb-1; K81; MLN137; Keratin,Hair,Basic 1; Hard Keratin Type II 1; Keratin, type II cuticular Hb1; Metastatic lymph node 137 gene protein

Specificity

This assay has high sensitivity and excellent specificity for detection of Keratin 81 (KRT81).
No significant cross-reactivity or interference between Keratin 81 (KRT81) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratin 81 (KRT81) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratin 81 (KRT81) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Transl Vis Sci Technol Association of Keratin 8 Level in Aqueous Humor With Outcomes of Intravitreal Ranibizumab Treatment for Neovascular Age-Related Macular Degeneration Pubmed:35040914
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPP892Hu04 Recombinant Keratin 81 (KRT81) Positive Control; Immunogen; SDS-PAGE; WB.
RPP892Hu02 Recombinant Keratin 81 (KRT81) Positive Control; Immunogen; SDS-PAGE; WB.
RPP892Hu01 Recombinant Keratin 81 (KRT81) Positive Control; Immunogen; SDS-PAGE; WB.
PAP892Hu01 Polyclonal Antibody to Keratin 81 (KRT81) WB; IHC; ICC; IP.
PAP892Hu02 Polyclonal Antibody to Keratin 81 (KRT81) WB; IHC; ICC; IP.
PAP892Hu04 Polyclonal Antibody to Keratin 81 (KRT81) WB; IHC; ICC; IP.
LAP892Hu71 Biotin-Linked Polyclonal Antibody to Keratin 81 (KRT81) WB; IHC; ICC.
SEP892Hu ELISA Kit for Keratin 81 (KRT81) Enzyme-linked immunosorbent assay for Antigen Detection.
LMP892Hu Multiplex Assay Kit for Keratin 81 (KRT81) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.