ELISA Kit for Keratin 33A (KRT33A)

KRTHA3A; Ha-3I; Krt1-3; K33A; HHA3-I; HKA3A; Keratin,Hair,Acidic 3A; Hard Keratin Type I 3I; Keratin, type I cuticular Ha3-I

Specificity

This assay has high sensitivity and excellent specificity for detection of Keratin 33A (KRT33A).
No significant cross-reactivity or interference between Keratin 33A (KRT33A) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratin 33A (KRT33A) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratin 33A (KRT33A) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPP899Hu01 Recombinant Keratin 33A (KRT33A) Positive Control; Immunogen; SDS-PAGE; WB.
PAP899Hu01 Polyclonal Antibody to Keratin 33A (KRT33A) WB,IHC,ICC/IF
MAP899Hu21 Monoclonal Antibody to Keratin 33A (KRT33A) WB; IHC; ICC; IP.
SEP899Hu ELISA Kit for Keratin 33A (KRT33A) Enzyme-linked immunosorbent assay for Antigen Detection.
LMP899Hu Multiplex Assay Kit for Keratin 33A (KRT33A) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.