Gene is regarded as the most important information carrier of organism. It stores all the genetic information of organisms, determines the biological characteristics, classification, life cycle and other characteristics. However, genes don’t directly join in the regulation of life activities. In higher organisms, DNA is served as template to get RNA by transcription, some RNA guide making protein by translation, the protein is directly joined in kinds of life activities. RNA play an important role connecting gene and protein. The RNA will directly or indirectly take part in the process of protein translation, gene regulation and nucleic acid cutting. All of these support that the stu......2017-07-07

Cells have to split the proliferation of the way. Single-celled organisms, which produce new members by cell division. Multicellular organisms, which produce new cells in a split way to supplement the aging or death cells in the body. Mitosis is the main mode for cell divisionof eukaryotes. Cell proliferation is the basis of organism growth, development, breeding, genetics, and so on, so cell proliferation detection has always been a hot research topic.Conventional cell proliferation detection techniques include the following:(1)Marker protein detection by WB and so on. PCNAis proliferating cell nuclear antigen, it plays an important role in starting DNA replication and cell prolifera......2017-07-07

It is known that the blood routing testsand one of them is C reactive protein(CRP) test. But who knows what CRP and its clinical significanceis?CRP is a protein which is produced by liver, ithas 224 amino acids and themonomer molecular mass of 25kDa. CRP can bind C polysaccharide on the surface of bacteria to form a complex to activate the classical complement pathway, which allows body to obtain the ability by nonspecific immune pathway to resist various bacteria, fungi and parasites, etc. In normal individuals, the CRP level in blood ranges from 800to 80000ug/L. And for organism with acute infection, serious injury or malignant tumor, the CRP level will rise dramatically in the next ......2017-07-07

Prothrombin Fragment 1+2(F1+2) is stable degradation product when the prothrombin is activated to thrombin. The F1+2 level in blood can reflect the activation level of thrombin in early stage, which can be used to diagnose the prethrombotic state & thrombotic diseases, remedy these diseases and monitor the efficacy.Take human F1+2 for example, the intrinsic and extrinsic pathways activate factor X to Xa, which removes the activation peptide and cleaves the remaining part of prothrombin into light and heavy chains by cutting the peptide bonds between Arg327-Thr328 and Arg363-Ile364 at same time, and then the F1+2(Ala44~Arg327) is released. While the thrombin can act on the prothrombin t......2017-06-12

As the development of engineered antibody, scFv (single-chain fragment variable) has attracted more and more attention in research of theoretical and practical applications. Compared to antibody, scFv is characteristic of small molecular weight, which accounted for 1/6 of MW of whole antibody. It is considered to be as the smallest functional unit with antibody biological activity.ScFv is a small engineered antibody in which the variable heavy chain(VH) and light chain (VL) of the antibody are connected by a short polypeptide linker with 15-20 amino acids. No Fc chain is included. ScFv was first found and expressed successfully in yeasts and plant cells, and it was able to spontaneousl......2017-05-19

It is well known that after knowing the gene sequence of the protein, the gene can be cloned into the plasmid. The plasmid can be transfected into E. coli, then the E.coli is the factory for protein expression. After separation and purification, the recombinant protein can be gotten. E. coli expression system is on of the expressions for protein expression. Eukaryotic expressin systems also can be used in protein expression. Figure 1 showed the expression of recombinant protein.Cloud-Clone Crop. provides one-stop services from genes to proteins. Customers also can choose one or two kinds of services according to their specific requirements. There are four protein expressions platform i......2017-05-19