Secondary antibodies are antibodies target to primary antibodies. The antibody (Ab), also known as an immunoglobulin, is able to provoke an immune response due to its immunogenicity. If an antibody is injected to heterogeneous host, the immunoglobulin against to this primary antibody will be secreted by activated immune system. A secondary antibody is used to combined with primary antibody that has bound to a target antigen and it can be used to increase sensitivity through the signal amplification. Secondary antibodies conjugated to enzymes and labels play an important role in the immunological experiment. That is to say, they are the key components of detection systems. Selection of ......2015-02-17

VEGF-A(Vascular Endothelial Growth Factor A), belongs to platelet derived growth factor family, mainly distributed in human and animal’s kidney, spleen, liver, skeleton, brain etc. VEGFA increases vascular permeability, promotes the multiplication and migration of vascular endothelial cell, decreases the tumor apoptosis, and enhances the generation of vascular support by binding with VEGFR1, VEGFR2, Nrp1 and Nrp2. VEGF-A mRNA consists of 8 exons and 7 introns, and yield 7 isoforms and 9 isomers by alternative splicing, mainly includes VEGF-A121, VEGF-A145, VEGF-A165, VEGF-A183, VEGF-A189 and VEGF-A206. The arabic number means the number of amino acids. The schematic diagram abou......2015-01-13

NF-κB(Nuclear Factor Kappa B) is a transcription factor, it is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet radiation, and bacterial or viral products. Activated NF-κB enters cell nucleus and promotes biological functional genes widely expressing. Under circumstances, more than 200 genes target NF-κB molecule in various types of cells. NF-κB not only functions in the regulation of immune and inflammatory response, but also in the proliferation, migration, and apoptosis. More and more evidences show that NF-kB plays an important role in neoplasia, and the abnormal expression of NF-kB has been detecte......2015-01-13

Generally, It needs about 4-5 hours for researchers to accomplish the experiment while using the common used sandwich ELISA kit. So, in order to improve the efficiency of research, Cloud-Clone Corp. had done lots of work in this area. Recently, the instant ELISA kit is pushed into the market. For instance, Instant ELISA Kit for Isocitrate Dehydrogenase 1, Soluble, (IDH1), the catalog number is IEH839Hu. Comparing to the normal ELISA kit for IDH1 (SEH839Hu), there are lots of advantages for the instant ELISA kit. For instance, the experimental time is short, sample volume needed for detection is small, reagents in the kit are ready to use, the sensitivity is high, et al. The comparison......2015-01-13

Heat shock proteins (heat shock protein, HSP) are a group of proteins rapidly induced by heat shock. An important feature in the biological world is that they are highly conserved in evolution. In recent years, HSP90a is the first one to be discovered and defined as the tumor marker by Chinese among more than 30 tumor biomarkers for tumor diagnosis worldwide. The others were defined by scientists in other countries. Cloud-Clone Corp. has concerned about this biological hotspot. It is well known that the HSPs are highly conserved. By aligning the sequences of the full length HSP90a from different species, such as human, rat, mouse, Guinea pig, pig, sheep, house, etc, the R&D Center ......2015-01-13

Anterior gradient protein 2 homolog (AGR2), a secretory protein localized in the endoplasmic reticulum, which was primary expressed in the trachea, lung, stomach, small intestine, colon and prostate. As it closely interacted with Mucin 2, ARG2 has been frequent mentioned in tumor related researches. Cloud-Clone Corp. has developed ARF2 protein, antibodies and series ELISA kits for researchers. AGR2 protein is encoded by 175 amino acids. After epitope analysis of functional domains, full length protein (Arg21~Leu175) excluding signal peptide sequences were chosen as the immunogen. This sequence retained whle antigenic epitopes, which would also be more convenient for researchers to con......2015-01-13