The assay principle of Competitive inhibition Elisa: coat specific antibody to solid carrier, then add antigen to be tested(standard and sample) and biotin-labeled antigen(detection reagent A). The tested antigen and biotin-labeled antigen competitively binds to immobile antibody. After incubation,the unbounded conjugate is washed off. Next, Horseradish Peroxidase (HRP)conjugated by avidin is added, the substrate solution TMB is added after incubation and washing.. TMB becomes blue under catalization of peroxidase, and finally turns to yellow after addition of acid. The higher concentration of tested sample is, the inhibition for bound of labeled antigen and antibody will be more obvio......2015-08-12

The stability of ELISA kit may refer to the ability to maintain the quality and efficiency of the kit during the shelf life defined by the Manufacturer. According to different experimental purpose and condition, Stability tests can be divided into accelerated stability tests and long-term stability test. The accelerated stability tests，is normally performed at 37℃ to accelerate the chemical or physical reaction of biological reagents in the kit. And then the stability of the kit is determined. Since many unexpected factors may exist during the transportation or storage, the stability of the kit under such conditions can be estimated by the accelerated stability test. And it can be......2015-08-12

For a sandwich ELISA test, the microtiter plate has been pre-coated with an antibody specific to analyte. Standards or samples are then added to the appropriate microtiter plate wells and the anaylte present is bound by the immobilized Ab. Next, Biotin conjugated to Detection Ab (Detection reagent A) is added and binds to the analyte absorbed on the plate. The binding of two antibodies and analytes in the wells act as a “sandwich” structure. After the unbound biotin conjugated to Detection Ab are washed away, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added and development is complete......2015-08-12

Besides for routine protein and antibody (polyclonal Ab and monoclonal Ab) custom services, based on our strong research team and served by the new bought fully automated peptidesynthesiser — aappptec Focus XC, Cloud-Clone Corp. provides new item for custom service program — Polypeptide Synthesis Service. What we can provide? Δ Quantity: More than 100 peptides per month Δ Time: About 10 days for one order Δ Length and purity: Regularly 6-25 amino acids, maximum up to 70 animo acids, with purity from 70% - 98% Δ Magnitude: From mg to g Δ Synthesizing method: Solid-phase and solution-phase synthesis Δ Labeling: FITC, HRP, Biotin la......2015-08-12

In March 2015, Cell Metabolism reported that MOTS-c, a 16-amino acid peptide encoded in the mitochondrial genome, plays an important role in metabolic homeostasis. From the research, it is found that MOTS-c mediates mitochondrial regulation of insulin through folate-purine-AMPK pathway. The discovery of MOTS-c provides new thoughts for age- and diet-dependent insulin resistance, diabetes and obesity. Moreover, the function of this peptide had been partially confirmed by mouse model (Fig.1.).The research of MOTS-c has becoming a hotspot after this initial report. In order to support the researches, we developed full suite of reagents for MOTS-c. Firstly, the peptide of MOTS-c (CPX132......2015-08-12

Small Molecules: Generally, small molecule refers to chemical compounds with low molecular weight (MW), lower than 1000 Dalton (Da). Different small molecules play distince roles in the pathology of clinical and scientific field, for instance the level of some hormones such as estron can be used as a biochemical indicator in a wide variety of infectious and noninfectious diseases. As a kind of typical hapten, small molecules lack immunogenicity which can trigger immunized animal to produce specific antibody. Thus, it is difficult for us to detect them quantified by conventional double-antibody ELISA method. However, as the development of molecule-coupling technology, plus our own modi......2015-08-12