Adenylosuccinate Synthase Like Protein 1 (ADSSL1)

ADSSL1 is a muscle isozyme of adenylosuccinate synthase, which catalyzes the initial reaction in the conversion of inosine monophosphate (IMP) to adenosine monophosphate (AMP) .

The ADSSL1 cDNA encodes a deduced 457-amino acid protein with a calculated molecular mass of approximately 50 kD that was predicted to be intracellular. ADSSL1 shares 96% amino acid identity with mouse Adss1, shares high similarity with other adenylosuccinate synthetases across many species, and contains a characteristic glycine-rich phosphate-binding loop and a guanine-specific binding region. RT-PCR detected ADSSL1 expression in all hematopoietic and solid tumor cell lines examined. Northern blot analysis of human tissues detected a 2.0-kb transcript, and a much weaker 4.4-kb transcript, in skeletal muscle and heart.