CLIA Kit for Alpha-1-Microglobulin (a1M)

AMBP; UTI; HCP; EDC1; HI30; IATIL; ITILC; ITI; ITIL; Alpha 1 Microglobulin/Bikunin Precursor; Growth-inhibiting protein 19; Uristatin; Uronic-Acid-Rich Protein; Trypstatin

Specificity

This assay has high sensitivity and excellent specificity for detection of Alpha-1-Microglobulin (a1M).
No significant cross-reactivity or interference between Alpha-1-Microglobulin (a1M) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Alpha-1-Microglobulin (a1M) and the recovery rates were calculated by comparing the measured value to the expected amount of Alpha-1-Microglobulin (a1M) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-105 99
EDTA plasma(n=5) 92-105 102
heparin plasma(n=5) 82-103 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-1-Microglobulin (a1M) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-1-Microglobulin (a1M) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-1-Microglobulin (a1M) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-104% 78-104% 89-96% 87-97%
EDTA plasma(n=5) 98-105% 78-103% 98-105% 95-102%
heparin plasma(n=5) 93-101% 79-99% 92-104% 84-98%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Diabetes Technology & Therapeutics Proteomic Identification of Human Urinary Biomarkers in Diabetes Mellitus Type 2 Liebert: 20100078
Journal of Pharmaceutical and Biomedical Analysis Effect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2 PubMed: 21130593
BioMed Research International Proteomic Profiling for Peritoneal Dialysate: Differential Protein Expression in Diabetes Mellitus PubMed: PMC3679811
Mainz Identifizierung neuer potentieller Biomarker für das Kolonkarzinom sowie dessen Vorstufen Info:Source
Cellular Physiology and Biochemistry Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory … Pubmed:29672286
Journal of Clinical Medicine Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients Pubmed: 30486327
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA217Hu61 Active Alpha-1-Microglobulin (a1M) Cell culture; Activity Assays.
APA217Hu62 Active Alpha-1-Microglobulin (a1M) Cell culture; Activity Assays.
EPA217Hu61 Eukaryotic Alpha-1-Microglobulin (a1M) Positive Control; Immunogen; SDS-PAGE; WB.
EPA217Hu62 Eukaryotic Alpha-1-Microglobulin (a1M) Positive Control; Immunogen; SDS-PAGE; WB.
RPA217Hu01 Recombinant Alpha-1-Microglobulin (a1M) Positive Control; Immunogen; SDS-PAGE; WB.
PAA217Hu01 Polyclonal Antibody to Alpha-1-Microglobulin (a1M) WB
LAA217Hu71 Biotin-Linked Polyclonal Antibody to Alpha-1-Microglobulin (a1M) WB; IHC; ICC.
MAA217Hu22 Monoclonal Antibody to Alpha-1-Microglobulin (a1M) WB; IHC; ICC; IP.
SEA217Hu ELISA Kit for Alpha-1-Microglobulin (a1M) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA217Hu CLIA Kit for Alpha-1-Microglobulin (a1M) Chemiluminescent immunoassay for Antigen Detection.
LMA217Hu Multiplex Assay Kit for Alpha-1-Microglobulin (a1M) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.