Virus, as a vector for exogenous gene espression, has been widely applied in many fields, including fucntional verification of gene, small RNA, transcription factor, recombinant protein production and transgenic animal research, et al. With the characteristic of virus infection and recombinant plasmid which contains viral genome, exogenous gene and others, protein overexpression or interference could be performed in mammalian cells.The technical system is mainly composed of two parts: virus packaging and virus infection (Fig1 and Fig 2). The virus packaging is the process of preparation recombinant plasmids by genetic engineering method. It can be divided into lentiviral packaging syst......2017-01-09

For immunoassay kit manufacturers, diagnostic reagent manufacturers, pharmaceutical factories which have large demands for detection reagents, the biggest obsession is the limited availability of raw materials. This knotty problem is smoothly solved as the antibody pairs were developed by Cloud-Clone Corp.. With high-quality antibody pairs, the efficient immunoassay detection assays can be developed, including ELISA, CLIA, ELISPOT, Luminex, Immunochromatography and other immunoassays.Here the reagents composition for antibody pairs are introduced in detail, shown in table 1. In addition, two common usages－sandwich ELISA and immunochromatography (colloid gold-immunochromatography assa......2017-01-09

The development of science and technology makes people to know the disease deeply. It was found that the etiology of many human diseases has an inseparable relationship with gene mutation. So, how to treat these diseases? People naturally think of re-editing mutant gene by using some techniques, this technology is gene editing technique. Based on protein/DNA identification and cut, gene editing techniques have been through the first generation of gene editing techniques, such as homing endonuclease, ZFN and TALEN. With the constantly understanding of the gene editing techniques, people realized the first generation of gene editing techniques have many defects, such as fussy operation, ......2017-01-09

Value-added products on the topic has continued for five, have you already had a general understanding of our value-added products? These series of topics’s writing are used to help our customers understand our product’s classification better, and find their own needs conveniently and accurately. Today we will continue to introduce three value-added products for everyone, followed by PCR Mix System, Lysis Buffer for Cells, Tissue, and Protein A/G Purification Column. The first one is PCR Mix System. PCR reagents are most widely used in bioscience research and almost all laboratories involved in molecular biology. At present, the Master Mix form of products is gradually becoming the......2016-12-15

The annual Nobel Prize holds as scheduled, and Japanese scientist Yoshinori Ohsumi was awarded this year’s Nobel Prize in Physiology or Medicine, by the discovery of cell autophagy mechanism. In the early 1950s, scientists in Belgium observed autophagic vacuole by electron microscope, and firstly put forward concept of "autophagy".Autophagy is a process of phagocytosing autologous cytoplasmic proteins or organelles, encapsulating them into vesicles and fusing with lysosomes, degrading the contents of lysosomes to achieve cellular homeostasis and organelle renewal. This procedure ended with a lysosomal closure chamber. Figure 1 graphically depicts the mechanism for autophagy......2016-12-15

Asprosin is a protein hormone that is produced by white adipose tissue in mammals, which is then transported to the liver and be stimulated for releasing of glucose into the blood stream. In the liver, asprosin activates rapid glucose release by a cAMP-dependent pathway. (Figure 1)Figure 1. Asprosin’s mode of action (Cited from cell ) This year, an article published in Cell reveals the discovery of Asprosin. The intention of this article is to study Neonatal Progeroid Syndrome (NPS), never thought in the process, they found a hormone - Asprosin, which is associated with type 2 diabetes. We will briefly introduce the discovery of this protein below. NPS is a genetic disorder, the pa......2016-12-15