With the consecutive improvement of life quality, there are more and more obese patients. According to the statistics, at least 2 million and 600 thousand people die of obesity each year. As one of the biggest killer of life, obesity can lead to a variety of metabolic diseases, including hypertension, coronary heart disease, diabetes and so on, which can seriously affect the health. In order to keep the obese people from disease, scientists pay more attention to research the mechanism and treatment of obesity. The AMPK signaling pathway can regulate the intracellular ATP supply as AMPK is an energy detector for intracellular ATP levels. Considering the central role of AMPK in regulatin......2016-12-15

Protein as the main executor of life activities,the research on its function and biological mechanism is explored in depth. So how to isolate and study a particular protein?The wide application of protein labeling technology can effectively solve this problem which makes many researchers get a headache. At present, some peptides and proteins are widely used for mass production of recombinant protein. In order to facilitate the protein expression, detection, tracing and purification, they are expressed with the target protein. This kind of polypeptide or protein is known as Protein Tag, such as MyC, His, GST, HA, etc.. The tag antibody can be highly specifically combined with the corres......2016-10-25

In last special subject we talked about the structural modification of small molecule haptenes, this part we will start discussing the choice of carrier proteins and coupling methods.Carrier protein is a kind of large molecule that can initiate immune reaction by itself, and it would be a totally artificial antigen after coupling of the carriers and small molecules. Carrier proteins commonly used include bovine serum albumin(BSA), keyhole limpet hemocyanin (KLH), ovalbumin(OVA), bovine thyroid globulin(BTG),etc.For selecting carrier protein, there are some main factors to be considered, such as its solubility, size, coupling groups, immunogenicity and cost price. At present bovine seru......2016-10-25

In vitro diagnosis(IVD) refers to the products and services that outside the human body, through detecting the human body samples (blood, body fluids, tissues, etc.) to obtain clinical diagnostic information, and then judge the disease or the body function. With the continuous development of science and technology, the in vitro diagnostic technique tends to be more and more simple, diversified, high throughput and precision. The development of in vitro diagnostic techniques is inseparable from the innovation of diagnostic reagents, which have gone through four technological revolution as chemical, enzyme, immunoassay and probe technology. Among them, the development of the immune detec......2016-10-25

CTXI is type I collagen cross-linking carboxyterminal telopeptide, type I collagen consists of three peptide chains, two of them are α(I) chains, the rest is α(Ⅱ) chain, three uncoiling short peptides exist in the carboxyl terminal of mature type I collagen. CTXI is a telopeptide which is disaggregated from the terminal of type I collagen. Due to the collagen with unique triplex super helical structure, its property is very stable, it cannot be resolved by the general processing temperature and short time heating, it is hard to be digestive absorption and not easy for the body to make full use of.Type I collagen mainly distributes in the skin, bone, cornea, muscle tendon and tumour......2016-09-13

To be prepared the antibodies against small molecules, the structure of small molecules is important, and it is commonly to have a certain complexity and rigidity, including benzene rings, heterocyclic groups or branched structures, otherwise, it would be difficult to produce antibodies with good titers.Some haptens have a reactive group, such as -COOH, -NH2, -OH, etc., and these haptens could be directly conjugated to a carrier protein, and then to be a totally artificial antigen, please see the the principle of coupling in Fig1.. However, the haptens should be re-designed and modified if there are no reactive groups which could be covalently conjugated to a carrier, or though the hap......2016-09-13