Precision means the testing consistency of measured values obtained by repeated test with same sample under same condition. As one of the basic analytical performance of the detection system, it is the basis of validity assessment for detection system. Well, how to measure the precision of ELISA kit?The precision of ELISA kit is divided into ‘Intra-assay Precision’ and ‘Inter-assay Precision’. It is shown in coefficient of variation(CV).Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level analytes were tested 20 times on one plate, respectively. Then the Mean and standard deviation(SD) were calculated by following formulas.Inter-assay Pre......2016-08-03

Tumor is a neoplasm which forms on the local tissue, due to the stimulation of various tumorigenic factors. It can be divided into benign and malignant tumor according to the biological characteristics of tumor cell and the damage degree to the body. Malignant tumor is also know as cancer, which is one of the main causes of death in the world nowadays. It has caused a serious hazard to human-beings, and restrict the social and economic development. Because of this, study of cancers has always been a hot spot in the field of biomedical research.Tumor markers refer to substances produced in malignant cells or host cells which is generated by the stimulation of malignant cells. It can ref......2016-08-03

Uromodulin is a glycoprotein encoded by the UMOD gene, which is the most abundant protein in urine of normal mammals. It’s mainly from the Heinz loop of the mammalian kidney.The molecular weight of monomer UMOD is about 85kDa. UMOD has several different isoforms and exists in aggregates, so the MW could reach millions Da. When the pH is low, precipitations may form.By the analysis of UMOD sequence, Glu28~Glu149 were chosen for gene cloning and protein expression to get recombinant protein, the cat no. is RPG918Hu01. Immunizing mouse and rabbit with the protein, we got the monoclonal antibody MAG918Hu21, polyclonal antibody PAG918Hu01, biotin labled polyclonal antibody PAG918Hu71. The......2016-06-12

Rheumatoid arthritis is a chronic autoimmune disease that is associated with complex inflammatory mediators, which will cause joint injury,synovial inflammation, cartilage and bone damage.The typical characteristic of this chronic disease is the chronic joint injury, seriously affecting people's health and the development of animal husbandry. The incidence of RA is generally believed to be caused by multiple factors,which include genetic factors,bacteria and virus mediated infection factors,autoimmune tolerance damage, endocrine disorders and other factors of cold,damp, fatigue, trauma, smoking and mental stimulation.The pathological symptoms were mainly manifested in the aspects o......2016-06-12

Chemiluminescence Enzyme Immunoassay is a kind of detection method which combined magnetic separation technology, sensitive chemiluminescence analysis with specific antigen-antibody reaction. For sandwich chemiluminescence enzyme immunoassay, the complex of enzyme labeled antibody, target antigen in samples and magnetic particle antibody act as a “sandwich” structure. And then the complex are segregated from other materials and unbound enzyme conjugated antibody. After that, the luminescent substrate is added to generate glow light emission kinetics. The target antigen can be measured qualitatively as the luminous intensity is proportional to the target antigen level in the sample ......2016-06-12

We had a brife introduction about the cascade activation signal pathway of MAPK in a early topic, including extracellular signal-regulated protein kinase(ERK), c-Jun N-terminal kinase(JNK), P38MAPK and ERK5. Among them, the pathways of JNK, P38MAPK and ERK5 belong to stress activated MAPK pathways. The discovery of ERK5 pathway is relatively late, but its research is very active.ERK5 includes 816 amino acids, and its length is almost 2 times of other members of the MAPK family. N-terminal of ERK5 has a domain of kinase, and C-terminal has a unique COOH, which is obviously different from the other members of the MAPK family. It is a three-dimensional structure of ERK5 shown in Figure 1.......2016-06-12