Interleukin 8 (IL8)

[Edit]

CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1

Interleukin 8 (IL8)

Interleukin 8 (IL-8), a member of the neutrophil-specific CXC subfamily of chemokines, is a potent neutrophil chemotactic and activating factor. It is a primary inflammatory cytokine produced by many cells in response to proinflammatory stimuli such as IL-1, TNF, LPS and viruses. Its function is, in part, to attract neutrophils to the site of inflammation and to activate them. The IL-8 cDNA sequence predicts a protein of 99 amino acids. Removal of a 22-residue signal peptide generates a mature protein of 77 amino acids (~ 8 kDa). Further proteolysis of the N-terminal end leads to a variant form with 72 amino acids; full activation of IL-8 may require cleavage to the 72 amino acid form. IL-8 can form non-covalent dimers in solution, especially at high concentrations, but dimerization is not necessary for biological activity.

Organism species: Homo sapiens (Human)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA080Hu01Recombinant Interleukin 8 (IL8)SDS-PAGE; WB; ELISA; IP.
APA080Hu01Active Interleukin 8 (IL8)Cell culture; Activity Assays; In vivo assays.
AntibodiesPAA080Hu01Polyclonal Antibody to Interleukin 8 (IL8)WB, ICC, IHC-P, IHC-F, ELISA
MAA080Hu22Monoclonal Antibody to Interleukin 8 (IL8)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
LAA080Hu82FITC-Linked Monoclonal Antibody to Interleukin 8 (IL8)IHC;ICC;IF;WB
LAA080Hu72Biotin-Linked Monoclonal Antibody to Interleukin 8 (IL8)IHC;WB;ELISA
LAA080Hu71Biotin-Linked Polyclonal Antibody to Interleukin 8 (IL8)IHC;WB;ELISA
LAA080Hu81FITC-Linked Polyclonal Antibody to Interleukin 8 (IL8)IHC;ICC;IF;WB
Assay KitsSEA080HuELISA Kit for Interleukin 8 (IL8)Enzyme-linked immunosorbent assay for Antigen Detection
SCA080HuCLIA Kit for Interleukin 8 (IL8)Chemiluminescent immunoassay for Antigen Detection
HEA080HuHigh Sensitive ELISA Kit for Interleukin 8 (IL8)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Interleukin 8 (IL8)Recombinant Protein Customized Service Offer
Antibodiesn/aMonoclonal Antibody to Interleukin 8 (IL8)Monoclonal Antibody Customized Service Offer
n/aPolyclonal Antibody to Interleukin 8 (IL8)Polyclonal Antibody Customized Service Offer
Assay Kitsn/aCLIA Kit for Interleukin 8 (IL8)CLIA Kit Customized Service Offer
n/aELISA Kit for Interleukin 8 (IL8)ELISA Kit Customized Service Offer

Organism species: Cavia (Guinea pig )

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA080Gu01Recombinant Interleukin 8 (IL8)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA080Gu01Polyclonal Antibody to Interleukin 8 (IL8)WB, ICC, IHC-P, IHC-F, ELISA
LAA080Gu71Biotin-Linked Polyclonal Antibody to Interleukin 8 (IL8)IHC;WB;ELISA
LAA080Gu81FITC-Linked Polyclonal Antibody to Interleukin 8 (IL8)IHC;ICC;IF;WB
MAA080Gu21Monoclonal Antibody to Interleukin 8 (IL8)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
Assay KitsSEA080GuELISA Kit for Interleukin 8 (IL8)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA080Rb01Recombinant Interleukin 8 (IL8)SDS-PAGE; WB; ELISA; IP.
RPA080Rb02Recombinant Interleukin 8 (IL8)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA080Rb52Polyclonal Antibody to Interleukin 8 (IL8)WB, ICC, IHC-P, IHC-F, ELISA
PAA080Rb51Polyclonal Antibody to Interleukin 8 (IL8)WB, ICC, IHC-P, IHC-F, ELISA
MAA080Rb22Monoclonal Antibody to Interleukin 8 (IL8)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
LAA080Rb71Biotin-Linked Polyclonal Antibody to Interleukin 8 (IL8)IHC;WB;ELISA
Assay KitsSEA080RbELISA Kit for Interleukin 8 (IL8)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA080Si01Recombinant Interleukin 8 (IL8)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA080Si01Polyclonal Antibody to Interleukin 8 (IL8)WB, ICC, IHC-P, IHC-F, ELISA
MAA080Si22Monoclonal Antibody to Interleukin 8 (IL8)WB, ICC, IHC-P, IHC-F, ELISA
LAA080Si71Biotin-Linked Polyclonal Antibody to Interleukin 8 (IL8)IHC;WB;ELISA
MAA080Si21Monoclonal Antibody to Interleukin 8 (IL8)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
Assay KitsSEA080SiELISA Kit for Interleukin 8 (IL8)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA080Ca01Recombinant Interleukin 8 (IL8)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA080Ca01Polyclonal Antibody to Interleukin 8 (IL8)WB, ICC, IHC-P, IHC-F, ELISA
MAA080Ca22Monoclonal Antibody to Interleukin 8 (IL8)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
LAA080Ca71Biotin-Linked Polyclonal Antibody to Interleukin 8 (IL8)IHC;WB;ELISA
LAA080Ca81FITC-Linked Polyclonal Antibody to Interleukin 8 (IL8)IHC;ICC;IF;WB
Assay KitsSEA080CaELISA Kit for Interleukin 8 (IL8)Enzyme-linked immunosorbent assay for Antigen Detection
SCA080CaCLIA Kit for Interleukin 8 (IL8)Chemiluminescent immunoassay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA080Po01Recombinant Interleukin 8 (IL8)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA080Po01Polyclonal Antibody to Interleukin 8 (IL8)WB, ICC, IHC-P, IHC-F, ELISA
MAA080Po22Monoclonal Antibody to Interleukin 8 (IL8)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
LAA080Po71Biotin-Linked Polyclonal Antibody to Interleukin 8 (IL8)IHC;WB;ELISA
LAA080Po81FITC-Linked Polyclonal Antibody to Interleukin 8 (IL8)IHC;ICC;IF;WB
Assay KitsSEA080PoELISA Kit for Interleukin 8 (IL8)Enzyme-linked immunosorbent assay for Antigen Detection
SCA080PoCLIA Kit for Interleukin 8 (IL8)Chemiluminescent immunoassay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA080Bo01Recombinant Interleukin 8 (IL8)SDS-PAGE; WB; ELISA; IP.
EPA080Bo61Eukaryotic Interleukin 8 (IL8)SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling.
APA080Bo61Active Interleukin 8 (IL8)Cell culture; Activity Assays; In vivo assays.
AntibodiesPAA080Bo01Polyclonal Antibody to Interleukin 8 (IL8)WB, ICC, IHC-P, IHC-F, ELISA
MAA080Bo21Monoclonal Antibody to Interleukin 8 (IL8)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEA080BoELISA Kit for Interleukin 8 (IL8)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Capra hircus; Caprine (Goat)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA080Cp01Recombinant Interleukin 8 (IL8)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA080Cp01Polyclonal Antibody to Interleukin 8 (IL8)WB, ICC, IHC-P, IHC-F, ELISA
Assay Kitsn/aCLIA Kit for Interleukin 8 (IL8)CLIA Kit Customized Service Offer
n/aELISA Kit for Interleukin 8 (IL8)ELISA Kit Customized Service Offer

Organism species: Equus caballus; Equine (Horse)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA080Eq01Recombinant Interleukin 8 (IL8)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA080Eq01Polyclonal Antibody to Interleukin 8 (IL8)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
LAA080Eq71Biotin-Linked Polyclonal Antibody to Interleukin 8 (IL8)IHC;WB;ELISA
MAA080Eq21Monoclonal Antibody to Interleukin 8 (IL8)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
Assay KitsSEA080EqELISA Kit for Interleukin 8 (IL8)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Chicken (Gallus)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA080Ga01Recombinant Interleukin 8 (IL8)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA080Ga01Polyclonal Antibody to Interleukin 8 (IL8)WB, ICC, IHC-P, IHC-F, ELISA
MAA080Ga22Monoclonal Antibody to Interleukin 8 (IL8)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
LAA080Ga71Biotin-Linked Polyclonal Antibody to Interleukin 8 (IL8)IHC;WB;ELISA
LAA080Ga81FITC-Linked Polyclonal Antibody to Interleukin 8 (IL8)WB; ICC; IHC-P; IHC-F; IF; ELISA.
Assay KitsSEA080GaELISA Kit for Interleukin 8 (IL8)Enzyme-linked immunosorbent assay for Antigen Detection
  1. "Induction of mRNA for a serine protease and a beta-thromboglobulin-like protein in mitogen-stimulated human leukocytes."J. Immunol. 139:250-256(1987) [PubMed] [Europe PMC] [Abstract]
  2. "Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor."J. Exp. Med. 167:1883-1893(1988) [PubMed] [Europe PMC] [Abstract]
  3. "Genomic structure of the human monocyte-derived neutrophil chemotactic factor IL-8."J. Immunol. 143:1366-1371(1989) [PubMed] [Europe PMC] [Abstract]
  4. "Regulation of the mRNA for monocyte-derived neutrophil-activating peptide in differentiating HL60 promyelocytes."Mol. Cell. Biol. 9:1946-1957(1989) [PubMed] [Europe PMC] [Abstract]
  5. "Coding region structure of interleukin-8 gene of human lung giant cell carcinoma LU65C cells that produce LUCT/interleukin-8: homogeneity in interleukin-8 genes."Immunol. Lett. 24:165-169(1990) [PubMed] [Europe PMC] [Abstract]
  6. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Purification of granulocyte chemotactic peptide/interleukin-8 reveals N-terminal sequence heterogeneity similar to that of beta-thromboglobulin."Eur. J. Biochem. 181:337-344(1989) [PubMed] [Europe PMC] [Abstract]
  9. "The neutrophil-activating proteins interleukin 8 and beta-thromboglobulin: in vitro and in vivo comparison of NH2-terminally processed forms."Eur. J. Immunol. 20:2113-2118(1990) [PubMed] [Europe PMC] [Abstract]
  10. "Three forms of monocyte-derived neutrophil chemotactic factor (MDNCF) distinguished by different lengths of the amino-terminal sequence."Mol. Immunol. 26:87-93(1989) [PubMed] [Europe PMC] [Abstract]
  11. "Purification and partial primary sequence of a chemotactic protein for polymorphonuclear leukocytes derived from human lung giant cell carcinoma LU65C cells."J. Exp. Med. 169:1895-1901(1989) [PubMed] [Europe PMC] [Abstract]
  12. "Biochemical and biological characterization of NAP-1/IL-8-related cytokines in lesional psoriatic scale."Adv. Exp. Med. Biol. 305:97-107(1991) [PubMed] [Europe PMC] [Abstract]
  13. "Inflammatory cytokines induce synthesis and secretion of gro protein and a neutrophil chemotactic factor but not beta 2-microglobulin in human synovial cells and fibroblasts."Biochem. J. 259:585-588(1989) [PubMed] [Europe PMC] [Abstract]
  14. "Isolation and amino acid sequence of a chemotactic protein, LECT/interleukin 8, from a human myeloid leukemia cell line, ML-1."Immunol. Lett. 36:71-81(1993) [PubMed] [Europe PMC] [Abstract]
  15. "Structure determination of a human lymphocyte derived neutrophil activating peptide (LYNAP)."Biochem. Biophys. Res. Commun. 151:883-890(1988) [PubMed] [Europe PMC] [Abstract]
  16. "Purification of a human monocyte-derived neutrophil chemotactic factor that has peptide sequence similarity to other host defense cytokines."Proc. Natl. Acad. Sci. U.S.A. 84:9233-9237(1987) [PubMed] [Europe PMC] [Abstract]
  17. "Purification and amino acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytes."Biochem. Biophys. Res. Commun. 149:755-761(1987) [PubMed] [Europe PMC] [Abstract]
  18. "Endothelial and leukocyte forms of IL-8. Conversion by thrombin and interactions with neutrophils."J. Immunol. 145:3033-3040(1990) [PubMed] [Europe PMC] [Abstract]
  19. "Chemical synthesis, purification, and characterization of two inflammatory proteins, neutrophil activating peptide 1 (interleukin-8) and neutrophil activating peptide."Biochemistry 30:3128-3135(1991) [PubMed] [Europe PMC] [Abstract]
  20. "Neutrophil gelatinase B potentiates interleukin-8 tenfold by aminoterminal processing, whereas it degrades CTAP-III, PF-4, and GRO-alpha and leaves RANTES and MCP-2 intact."Blood 96:2673-2681(2000) [PubMed] [Europe PMC] [Abstract]
  21. "Identification of biologically active chemokine isoforms from ascitic fluid and elevated levels of CCL18/pulmonary and activation-regulated chemokine in ovarian carcinoma."J. Biol. Chem. 277:24584-24593(2002) [PubMed] [Europe PMC] [Abstract]
  22. "Interleukin-8, a chemotactic and inflammatory cytokine."FEBS Lett. 307:97-101(1992) [PubMed] [Europe PMC] [Abstract]
  23. "Regulation of the immune response by the interaction of chemokines and proteases."Adv. Immunol. 81:1-44(2003) [PubMed] [Europe PMC] [Abstract]
  24. "Citrullination of CXCL8 by peptidylarginine deiminase alters receptor usage, prevents proteolysis, and dampens tissue inflammation."J. Exp. Med. 205:2085-2097(2008) [PubMed] [Europe PMC] [Abstract]
  25. "Citrullination of CXCL8 increases this chemokine's ability to mobilize neutrophils into the blood circulation."Haematologica 94:1346-1353(2009) [PubMed] [Europe PMC] [Abstract]
  26. "Endoplasmic reticulum stress-activated C/EBP homologous protein enhances nuclear factor-kappaB signals via repression of peroxisome proliferator-activated receptor gamma."J. Biol. Chem. 285:35330-35339(2010) [PubMed] [Europe PMC] [Abstract]
  27. "Determination of the secondary structure of interleukin-8 by nuclear magnetic resonance spectroscopy."J. Biol. Chem. 264:18907-18911(1989) [PubMed] [Europe PMC] [Abstract]
  28. "Three-dimensional structure of interleukin 8 in solution."Biochemistry 29:1689-1696(1990) [PubMed] [Europe PMC] [Abstract]
  29. "Structure and activity of a chimeric interleukin-8-melanoma-growth-stimulatory-activity protein."Eur. J. Biochem. 235:26-35(1996) [PubMed] [Europe PMC] [Abstract]
  30. "Structure of a CXC chemokine-receptor fragment in complex with interleukin-8."Structure 7:157-168(1999) [PubMed] [Europe PMC] [Abstract]
  31. "Crystallization of human interleukin-8. A protein chemotactic for neutrophils and T-lymphocytes."J. Biol. Chem. 265:6851-6853(1990) [PubMed] [Europe PMC] [Abstract]
  32. "Comparison of the solution nuclear magnetic resonance and crystal structures of interleukin-8. Possible implications for the mechanism of receptor binding."J. Mol. Biol. 217:611-620(1991) [PubMed] [Europe PMC] [Abstract]
  33. "Crystal structure of interleukin 8: symbiosis of NMR and crystallography."Proc. Natl. Acad. Sci. U.S.A. 88:502-506(1991) [PubMed] [Europe PMC] [Abstract]
  34. "Receptor-binding conformation of the 'ELR' motif of IL-8: X-ray structure of the L5C/H33C variant at 2.35 A resolution."Proteins 38:361-367(2000) [PubMed] [Europe PMC] [Abstract]