• Star-Product
ELISA Kit for Interleukin 8 (IL8)

CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1

Specificity

This assay has high sensitivity and excellent specificity for detection of Interleukin 8 (IL8).
No significant cross-reactivity or interference between Interleukin 8 (IL8) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 8 (IL8) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 8 (IL8) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-103 96
EDTA plasma(n=5) 87-94 91
heparin plasma(n=5) 97-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 8 (IL8) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-101% 88-102% 99-105% 94-103%
EDTA plasma(n=5) 91-105% 85-101% 88-95% 92-101%
heparin plasma(n=5) 86-101% 89-99% 94-101% 81-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Reproductive Biology and Endocrinology Evidence that polymorphonuclear neutrophils infiltrate into the developing corpus luteum and promote angiogenesis with interleukin-8 in the cow Rbej: 14777827
Acta Otolaryngol Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa PubMed: 19958244
Journal of Clinical Periodontology Experimental peri-implant mucositis at different implant surfaces. Pubmed: 24521508
Physiological Research Budesonide added to modified porcine surfactant Curosurf may additionally improve the lung functions in meconium aspiration syndrome. Pubmed: 2432969
Rheumatology International Serum levels of selected chemokines in systemic lupus erythematosus patients Pubmed: 22461186
J Med Life. Expression of interleukine-8 as an independent prognostic factor for sporadic colon cancer dissemination Pubmed:Pmc4197484
J Clin Periodontol. Experimental peri‐implant mucositis at different implant surfaces Pubmed:24521508
British Journal of Nutrition Grape seed extract supplementation attenuates the heat stress-induced responses of jejunum epithelial cells in Simmental?×?Qinchuan steers Pubmed:24846452
Theriogenology Inflammatory cytokine concentrations in uterine flush and serum samples from dairy cows with clinical or subclinical endometritis Theriojournal:Source
Anim Reprod Sci. Alteration in peripheral blood concentration of certain pro-inflammatory cytokines in cows developing retention of fetal membranes PubMed: 25851495
Медицина и здравоохранение ДИНАМИКА ЦИТОКИНОВОГО ПРОФИЛЯ В ОЦЕНКЕ ЭФФЕКТИВНОСТИ РАЗНЫХ МЕТОДОВ СТИМУЛЯЦИИ РЕПАРАТИВНОГО ОСТЕОГЕНЕЗА В ЭКСПЕРИМЕНТЕ Article: N
BMC Anesthesiology Hyperinflation deteriorates arterial oxygenation and lung injury in a rabbit model of ARDS with repeated open endotracheal suctioning PubMed: 25943099
J Breath Res Effects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgery PubMed: 25719610
ARTICLE The Predictive Diagnostic and Prognostic Cut-off Values for Interleukin 8 in Patients with Meningitis in Egypt Profile: Mohmad_Mosaad
Veterinary Immunology and Immunopathology Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period Pubmed:27090626
Infection, Genetics and Evolution Infection of chicken bone marrow mononuclear cells with subgroup J avian leukosis virus inhibits dendritic cell differentiation and alters cytokine expression Pubmed:27349993
Asian Pacific Journal of Tropical Medicine Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injury science:S1995764516300955
Archives of Biological Sciences Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol publication:299492265_Diabetes-induced_renal_failu
Journal of Agricultural Biotechnology Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells 10610
journal of molecular and cellular cardiology Mutant DD genotype of NFKB1 gene is associated with the susceptibility and severity of coronary artery disease. pubmed:28088561
Physiological Research Intravenous dexamethasone attenuated inflammation and influenced apoptosis of lung cells in an experimental model of acute lung injury. pubmed:28006948
PLOS ONE Mechanical Ventilation Alters the Development of Staphylococcus aureus Pneumonia in Rabbit. pubmed:27391952
The Journal of Comenius University in Bratislava Effects of Conventional Mechanical Ventilation Performed by Two Neonatal Ventilators on the Lung Functions of Rabbits with Meconium-Induced Acute Lung Injury acm.2017.16.issue-3
Journal of Physiology and Pharmacology Effects of budesonide on the lung functions, inflammation and apoptosis in a saline-lavagemodel of acute lung injury. pubmed:28195073
中国药物警戒 小剂量阿奇霉素对稳定期老年慢性阻塞性肺疾病患者外周血内TNF-α、IL-8、CRP水平及肺功能的影响 Periodical_zgywjj201608004
Veterinary Immunology and Immunopathology Cytokine levels in colostrum and in foals' serum pre- and post-suckling pubmed:28242000
Catalog No. Related products for research use of Canis familiaris; Canine (Dog) Organism species Applications (RESEARCH USE ONLY!)
RPA080Ca01 Recombinant Interleukin 8 (IL8) Positive Control; Immunogen; SDS-PAGE; WB.
PAA080Ca01 Polyclonal Antibody to Interleukin 8 (IL8) WB; IHC; ICC; IP.
LAA080Ca71 Biotin-Linked Polyclonal Antibody to Interleukin 8 (IL8) WB; IHC; ICC.
LAA080Ca81 FITC-Linked Polyclonal Antibody to Interleukin 8 (IL8) WB; IHC; ICC; IF.
MAA080Ca22 Monoclonal Antibody to Interleukin 8 (IL8) WB; IHC; ICC; IP.
SEA080Ca ELISA Kit for Interleukin 8 (IL8) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA080Ca Instant ELISA Kit for Interleukin 8 (IL8) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA080Ca CLIA Kit for Interleukin 8 (IL8) Chemiluminescent immunoassay for Antigen Detection.
KSA080Ca01 ELISA Kit DIY Materials for Interleukin 8 (IL8) Main materials for "Do It (ELISA Kit) Yourself".