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Active Bone Morphogenetic Protein Receptor 2 (BMPR2)

BMPRII; BMPR-II; BRK3; T-ALK; BMPR3; PPH1; Primary Pulmonary Hypertension 1; BMP type II receptor

ACTIVITY TEST

Bone Morphogenetic Protein Receptor 2 (BMPR2) is a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. BMPR2 is a TGFβ type II receptor expressed on cumulus cells. Growth Differentiation Factor 9 (GDF9) can bind to BMPR2 through different type I receptors, thereby regulating downstream metabolic reactions. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human BMPR2 and recombinant human GDF9. Briefly, biotin-linked BMPR2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to GDF9-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human BMPR2 and recombinant human GDF9 was shown in Figure 1, the EC50 for this effect is 42.87 ng/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Biomedicines Participation of Selected Soluble BMP-2 and BMP-7 Bone Morphogenetic Proteins and Their Soluble Type IALK-1 and Type IIBMPR2 Receptors in Formation and … 34680408
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