≡

Active Interleukin 11 (IL11)

AGIF; Adipogenesis Inhibitory Factor; Oprelvekin

ACTIVITY TEST

Interleukin 11 (IL-11) is a multifunctional cytokine. It is a key regulator of multiple events in hematopoiesis, most notably the stimulation of megakaryocyte maturation. IL-11 has been demonstrated to improve platelet recovery after chemotherapy-induced thrombocytopenia, induce acute phase proteins, modulate antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation IL-11 causes bone-resorption. Besides, IL-11 have been proved can promote migration of A549 cells, 5×104 cells were seeded into 6 well plates. After cell confluent, replace with DMEM without serum overnight. Using a (yellow) pipette tip make a straight scratch, simulating a wound, then washing the wells three times with PBS. Adding 1% serum standard DMEM containing various concentrations of recombinan human IL-11 to each well, incubating the plate for 48 hours at 37℃, 5% CO2. The results observed by inverted microscope was shown in Figure.
(A) A549 cells cultured in DMEM with 100ng/mL IL-11 for 48h;
(B) A549 cells cultured in DMEM before addition IL-11.
Figure. Wound healing assay of A549 cells after stimulated with IL-11.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Molecular Medicine Reports Regulatory effect of chemerin and therapeutic efficacy of chemerin‑9 in pancreatogenic diabetes mellitus Pubmed: 31922216
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
EPA057Hu61 Eukaryotic Interleukin 11 (IL11) Positive Control; Immunogen; SDS-PAGE; WB.
APA057Hu01 Active Interleukin 11 (IL11) Cell culture; Activity Assays.
EPA057Hu62 Eukaryotic Interleukin 11 (IL11) Positive Control; Immunogen; SDS-PAGE; WB.
RPA057Hu01 Recombinant Interleukin 11 (IL11) Positive Control; Immunogen; SDS-PAGE; WB.
APA057Hu62 Active Interleukin 11 (IL11) Cell culture; Activity Assays.
APA057Hu61 Active Interleukin 11 (IL11) Cell culture; Activity Assays.
PAA057Hu01 Polyclonal Antibody to Interleukin 11 (IL11) WB; IHC; ICC; IP.
MAA057Hu22 Monoclonal Antibody to Interleukin 11 (IL11) WB; IHC; ICC; IP.
MAA057Hu21 Monoclonal Antibody to Interleukin 11 (IL11) WB; IHC; ICC; IP.
SEA057Hu ELISA Kit for Interleukin 11 (IL11) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA057Hu Multiplex Assay Kit for Interleukin 11 (IL11) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.