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Active Growth Arrest Specific Protein 6 (GAS6)

AXLLG; AXSF; AXL Stimulatory Factor; AXL receptor tyrosine kinase ligand

ACTIVITY TEST

Growth arrest-specific 6, also known as GAS6, is a gamma-carboxyglutamic acid (Gla) domain-containing protein thought to be involved in the stimulation of cell proliferation. It has been reported that both PC-3 and DU 145 human prostate cancer cell lines are stimulated to proliferate by Gas6, however, this proliferative response strictly correlates with the expression of the Axl receptor, being higher in DU 145 cells. To test the proliferative effect of Gas6, DU 145 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of GAS6. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC.
Cell proliferation of DU145 cells after incubation with GAS6 for 72h observed by inverted microscope was shown in Figure 1.

The dose-effect curve of GAS6 was shown in Figure 2. It was obvious that GAS6 significantly promoted cell proliferation of DU145 cells. The ED50 for this effect is typically 0.77~38.08ng/mL.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Acta Pharmacol Sin. Pharmacological evidence: a new therapeutic approach to the treatment of chronic heart failure through SUR2B/Kir6.1 channel in endothelial cells. pubmed:27890915
Frontiers of Medicine The effects of Parenteral K1 administration in Pseudoxanthoma elasticum Patients Versus controls. a Pilot study Pubmed:29713628
Oncotarget DAPK and CIP2A are involved in GAS6/AXL-mediated Schwann cell proliferation in a rat model of bilateral cavernous nerve injury Pubmed:29464081
journal of nutritional biochemistry Gamma-glutamyl carboxylated Gas6 mediates the beneficial effect of vitamin K on lowering hyperlipidemia via regulating the AMPK/SREBP1/PPARα signaling … Pubmed: 31226525
CELL DEATH AND DIFFERENTIATION KPNB1-mediated nuclear translocation of PD-L1 promotes non-small cell lung cancer cell proliferation via the Gas6/MerTK signaling pathway Pubmed: 33139930
重度子痫前期患者血清GAS6 与其他炎症因子的相关性研究
JOURNAL OF NUTRITIONAL BIOCHEMISTRY Gamma-glutamyl carboxylated Gas6 facilitates the prophylactic effect of vitamin K in inhibiting hyperlipidemia-associated inflammatory pathophysiology via arresting MCP-1/ICAM-1 mediated monocyte-hepatocyte adhesion 33789149
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA204Hu01 Active Growth Arrest Specific Protein 6 (GAS6) Cell culture; Activity Assays.
RPA204Hu01 Recombinant Growth Arrest Specific Protein 6 (GAS6) Positive Control; Immunogen; SDS-PAGE; WB.
RPA204Hu02 Recombinant Growth Arrest Specific Protein 6 (GAS6) Positive Control; Immunogen; SDS-PAGE; WB.
PAA204Hu02 Polyclonal Antibody to Growth Arrest Specific Protein 6 (GAS6) WB; IHC; ICC; IP.
PAA204Hu03 Polyclonal Antibody to Growth Arrest Specific Protein 6 (GAS6) WB; IHC; ICC; IP.
PAA204Hu01 Polyclonal Antibody to Growth Arrest Specific Protein 6 (GAS6) WB; IHC; ICC; IP.
LAA204Hu71 Biotin-Linked Polyclonal Antibody to Growth Arrest Specific Protein 6 (GAS6) WB; IHC; ICC.
MAA204Hu22 Monoclonal Antibody to Growth Arrest Specific Protein 6 (GAS6) WB; IHC; ICC; IP.
SEA204Hu ELISA Kit for Growth Arrest Specific Protein 6 (GAS6) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA204Hu Multiplex Assay Kit for Growth Arrest Specific Protein 6 (GAS6) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.