≡

Active Adrenomedullin (ADM)

AM; PAMP; Proadrenomedullin N-20 terminal peptide; ProAM N-terminal 20 peptide

ACTIVITY TEST

Adrenomedullin (ADM) is a peptide hormone first discovered in 1993 in pheochromocytoma. Although AM is highly expressed in the adrenal glands, heart, lungs, and kidneys, vascular endothelium and smooth muscle are thought to be the main source of plasma AM. It is a 52 aa peptide with several functions, including vasodilation, regulation of hormone secretion, promotion of angiogenesis, and antimicrobial activity. The antimicrobial activity is antibacterial, as the peptide has been shown to kill E. coli and S. aureus at low concentration. Receptor activity-modifying protein 2 can transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for adrenomedullin (AM) together with CALCRL. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse ADM and recombinant human RAMP2. Briefly, ADM was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to RAMP2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ADM pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse ADM and recombinant human RAMP2 was shown in Figure 1, the EC50 for this effect is 0.16 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Periodontal Research Gingival crevicular fluid adrenomedullin level in individuals with and without diabetes mellitus type 2 Wiley: Source
J Periodontal Res. Comparison of peri-implant crevicular fluid levels of adrenomedullin and human beta defensins 1 and 2 from mandibular implants with different implant stability quotient levels in nonsmoker patients Pubmed: 23898869
Journal of Biosciences Short-term hypoxia/reoxygenation activates the angiogenic pathway in rat caudate putamen. Pubmed: 23660671
Journal of Translational Medicine DNA methylation patterns in newborns exposed to tobacco in utero Pubmed:25623364
Pediatric Hematology and Oncology Adrenomedullin—A New Marker in Febrile Neutropenia: Comparison With CRP and Procalcitonin PubMed: 26271020
Biogerontology. Angiogenesis is VEGF-independent in the aged striatum of male rats exposed to acute hypoxia. pubmed:28501895
Journal of Clinical Investigation  Adrenomedullin and Galanin Responses to Orthostasis in Older Persons pubmed:28796366
Clinical Pathology and Medical Laboratory ADRENOMEDULIN DI KARSINOMA PAYUDARA DENGAN METASTASIS :
Phytomedicine Composition and cardioprotective effects of Primula veris L. solid herbal extract in experimental chronic heart failure Doi: 10.1016/j.phymed.2018.09.015
Biomedical Journal of Scientific & Technical Research Anti-Inflammatory and Pro-Inflammatory Neuropeptides in Dilative Cardiomyopathy are there Relations to Psychophysical Stress?
博士论文 Clinical and experimental investigation of the nervous system of neonatal foals. Neuroendocrin transition from foetal consciousness to perinatal life
Biology Hemodynamic Responses in Lower Limb Lymphedema Patients Undergoing Physical Therapy 34356497
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APA220Mu01 Active Adrenomedullin (ADM) Cell culture; Activity Assays.
RPA220Mu02 Recombinant Adrenomedullin (ADM) Positive Control; Immunogen; SDS-PAGE; WB.
RPA220Mu01 Recombinant Adrenomedullin (ADM) Positive Control; Immunogen; SDS-PAGE; WB.
PAA220Mu02 Polyclonal Antibody to Adrenomedullin (ADM) WB; IHC; ICC; IP.
PAA220Mu01 Polyclonal Antibody to Adrenomedullin (ADM) WB; IHC; ICC; IP.
CEA220Mu ELISA Kit for Adrenomedullin (ADM) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA220Mu Multiplex Assay Kit for Adrenomedullin (ADM) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.