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Active Vitamin D Receptor (VDR)

NR1I1; Nuclear Receptor Subfamily 1 Group I Member 1; Calcitriol Receptor; 1,25-dihydroxyvitamin D3 receptor

ACTIVITY TEST

Vitamin D Receptor (VDR) is the nuclear hormone receptor for vitamin D3. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. It mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Besides, Retinoid X Receptor Alpha (RXRa) has been identified as an interactor of VDR, thus a binding ELISA assay was conducted to detect the interaction of recombinant human VDR and recombinant human RXRa. Briefly, VDR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to RXRa-coated microtiter wells and incubated for 1h at 37℃. Wells were aspirated and incubated for 1h with anti-VDR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450nm immediately. The binding activity of of VDR and RXRa was shown in Figure 1, and this effect was in a dose dependent manner. The EC50 for this effect is 0.469 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
6 The vitamin D receptor: A therapeutic target for the treatment of breast cancer? Cgi: Content
endocrine-related cancer Vitamin D receptor as a target for breast cancer therapy. pubmed:28213567
Journal of Advanced Research Effect of exercise on serum vitamin D and tissue vitamin Dreceptors in experimentally induced type 2 Diabetes Mellitus. pubmed:27504197
European Journal of Applied Physiology Vitamin D supplementation attenuates oxidative stress in paraspinal skeletal muscles in patients with low back pain Pubmed:29143122
BBA- Molecular Basis of Disease Enhanced remedial effects for vitamin D3 and calcium co-supplementation against pre-existing lead nephrotoxicity in mice: The roles of renal calcium homeostatic … Pubmed: 30553018
OPEN ACCESS MACEDONIAN JOURNAL OF MEDICAL SCIENCES Seluang Fish (Rasbora Spp.) Oil Decreases Inflammatory Cytokines Via Increasing Vitamin D Level in Systemic Lupus Erythematosus
Journal of Steroid Biochemistry and Molecular Biology Status of vitamin D and the associated host factors in pulmonary tuberculosis patients and their household contacts: a cross sectional study Pubmed: 31255688
Scientific Reports Association of Fok1 VDR polymorphism with Vitamin D and its associated molecules in pulmonary tuberculosis patients and their household contacts Pubmed: 31649297
Research square Vitamin D Status in Dupuytren's Disease: Association with Clinical Status and Vitamin D Receptor Expression
Betok Fish (Anabas testudineus) Oil Decreases Inflammatory Cytokine through Increasing Vitamin D Level in Rats-induced Systemic Lupus Erythematosus
iium medical journal malaysia Positive Correlation between Monocyte-to-Lymphocyte Ratio and C-Reactive Protein in Vitamin D Deficient Preterm Infants with Respiratory Distress Syndrome
Research Square Identification of SNP of VDR and VDBP gene and its Dysregulated pathway through VDR-VDBP interaction network analysis in Vitamin D deficient Infertile …
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA475Hu01 Active Vitamin D Receptor (VDR) Cell culture; Activity Assays.
RPA475Hu01 Recombinant Vitamin D Receptor (VDR) Positive Control; Immunogen; SDS-PAGE; WB.
PAA475Hu01 Polyclonal Antibody to Vitamin D Receptor (VDR) WB
MAA475Hu22 Monoclonal Antibody to Vitamin D Receptor (VDR) WB; IHC; ICC; IP.
SEA475Hu ELISA Kit for Vitamin D Receptor (VDR) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA475Hu Multiplex Assay Kit for Vitamin D Receptor (VDR) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.