≡

Active Vitronectin (VTN)

V75; VN; VNT; Serum Spreading Factor; Somatomedin B; Complement S-Protein

ACTIVITY TEST

Vitronectin (VTN ) is a glycoprotein of the hemopexin family which is abundantly found in serum, the extracellular matrix and bone. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. VTN can binds to integrin alpha-V beta-3 and thus promotes cell adhesion and spreading. It also binds to plasminogen activator inhibitor-1 (PAI-1) and serves to regulate proteolysis initiated by plasminogen activation. Besides, Insulin Like Growth Factor Binding Protein 5 (IGFBP5) has been identified as an interactor of VTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant Rat VTN and recombinant Rat IGFBP5. Briefly, VTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to IGFBP5-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-VTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of VTN and IGFBP5 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Biomedical Engineering and Informatics Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer IEEE: 6098563
PLoS ONE Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical Significance PubMed: PMC3754973
Liver International Identification of candidate biomarkers for hepatocellular carcinoma in plasma of HCV-infected cirrhotic patients by 2-D DIGE Pubmed: 23944848
Analytical Biochemistry A multianalytical approach to investigate the effect of nanofiltration on plasma-derived factor IX clinical lots Pubmed:29154788
Journal of Materials Chemistry B Serum protein adsorption profile on BCP ceramic and influence of the elevated adsorption of adhesive proteins on the behaviour of MSCs Doi: 10.1039/C8TB02283F 
JOURNAL OF NEPHROLOGY Urinary vitronectin identifies patients with high levels of fibrosis in kidney grafts 33275196
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB041Ra01 Recombinant Vitronectin (VTN) Positive Control; Immunogen; SDS-PAGE; WB.
APB041Ra01 Active Vitronectin (VTN) Cell culture; Activity Assays.
PAB041Ra01 Polyclonal Antibody to Vitronectin (VTN) WB; IHC; ICC; IP.
MAB041Ra21 Monoclonal Antibody to Vitronectin (VTN) WB; IHC; ICC; IP.
SEB041Ra ELISA Kit for Vitronectin (VTN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB041Ra Multiplex Assay Kit for Vitronectin (VTN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB041Ra01 ELISA Kit DIY Materials for Vitronectin (VTN) Main materials for "Do It (ELISA Kit) Yourself".