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Active Alkaline Phosphatase, Tissue-nonspecific (ALPL)
BALP; BSAP; HOPS; AP-TNAP; TNSALP; Bone Alkaline Phosphatase; Alkaline Phosphatase,Tissue-Nonspecific Isozyme; Alkaline Phosphatase, Liver/Bone/Kidney
- Product No.APB091Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point6.7
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
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For more details, please contact local distributors!
ACTIVITY TEST
Four distinct genes encode alkaline phosphatases (APs) in humans. The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissue nonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers. There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones. The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The activity assay of recombinant human ALPL was measured by its ability to cleave a peptide substrate, 4-Methumbelliferly phosphate. The reaction was performed in 50 mM Tris, 1 mM MgCl2, pH 9.0 ( assay buffer), ainitiated by addition 50 μL of 0.625 ug/ml ALPL (diluted by assay buffer) to 50 µL of 50 uM substrate. Read at excitation and emission wavelengths of 365 nm and 445 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human ALPL is >11000 pmol/min/µg.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Journal of Materials Science: Materials in Medicine | Osteopenic bone cell response to strontium-substituted hydroxyapatite SpringerLink: n8233337w171v451 |
| Osteoporosis International | Dextromethorphan inhibits osteoclast differentiation by suppressing RANKL-induced nuclear factor-κB activation PubMed: 23400250 |
| Journal of Applied Toxicology | Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in mice Wiley: Source |
| Acta Pharmacologica Sinica | The antidepressant bupropion exerts alleviating properties in an ovariectomized osteoporotic rat model Pubmed:25544359 |
| Biomedical Materials | Enhanced differentiation of osteoblastic cells on novel chitosan/β-1, 3-glucan/bioceramic scaffolds for bone tissue regeneration Pubmed:25586067 |
| Pharmacology | Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy PubMed: 25871861 |
| J Biomed Mater Res A. | New method for the fabrication of highly osteoconductive β-1,3-glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological characterization. Pubmed:27239050 |
| J Bone Miner Metab | MiR-142-5p promotes bone repair by maintaining osteoblast activity Pubmed:27085967 |
| Journal of Biomedical Materials Research | New method for the fabrication of highly osteoconductive β‐1, 3‐glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological … doi:10.1002 |
| Journal of Bone and Mineral Metabolism | MiR‑142‑5p promotes bone repair by maintaining osteoblast activity pubmed:27085967 |
| Scientific Reports | Hydrolysis of Extracellular Pyrophosphate increases in post-hemodialysis plasma Pubmed:30038263 |
| International Journal of Molecular Sciences | Suppression Effect of Astaxanthin on Osteoclast Formation In Vitro and Bone Loss In Vivo Pubmed:29562730 |
| Pharmacology | Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats Pubmed:29898457 |
| Experimental and Therapeutic Medicine | Combination therapy with BMP‑2 and psoralen enhances fracture healing in ovariectomized mice 10.3892:etm.2018.6353 |
| American Journal of Translational Research | Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9 |
| The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro | |
| International Journal of Biomaterials | Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect 34104195 |