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Active Netrin 1 (Ntn1)

NTN1L; Epididymis tissue protein Li 131P

ACTIVITY TEST

Netrin 1 (Ntn1) is included in a family of laminin-related secreted proteins. Mouse Ntn1 is a 66-76 kDa glycoprotein that is well known for its involvement in axonal guidance during embryonic development and as an enhancer of cancer cell metastasis. Ntn1 is also involved in outgrowth and migration orientation in the developing CNS and plays a significant role in the morphogenesis of endothelial cells and vascular smooth-muscle cells. It is also involved in the processes of cytoskeleton reorganization, angiogenesis, epithelial cell adhesion, and cell migration in the lungs, mammary gland, and pancreas. Slit Homolog 1 (Slit1) can acts as a potent promoter of both Netrin-1 attractive and repulsive activities on distinct neuronal cell types, thereby opening novel perspectives on the role of combinations of cues in brain wiring. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse Ntn1 and recombinant human Slit1. Briefly, Ntn1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to Slit1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-Ntn1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse Ntn1 and recombinant human Slit1 was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Transplantation Proceedings Netrin-1: A Novel Universal Biomarker of Human Kidney Injury PubMed: 20620466
Biomarkers Plasma netrin-1 is a diagnostic biomarker of human cancers PubMed: PMC3143477
Journal of Maternal-Fetal and Neonatal Medicine Cord blood netrin-1 and -4 concentrations in term pregnancies with normal, restricted and increased fetal growth Informahealthcare: Source
4 Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity Pubmed:24584118
J Matern?Fetal?Neonatal Med.? Cord blood netrin-1 and-4 concentrations in term pregnancies with normal, restricted and increased fetal growth Pubmed:24716747
Renal failure. Urinary netrin-1 and KIM-1 as early biomarkers for septic acute kidney injury Pubmed:25154466
Stem Cell Research & Therapy Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton’s jelly mesenchymal stem cells (WJ-MSC) articles:10.1186
Oncotarget The effect of aspirin on circulating netrin-1 levels in humans is dependent on the inflammatory status of the vascular endothelium. pubmed:29156815
Frontiers in Cellular Neuroscience Neuroprotection Exerted by Netrin-1 and Kinesin Motor KIF1A in Secondary Brain Injury following Experimental Intracerebral Hemorrhage in Rats Pubmed:29375318
Scientific Reports Netrin-1 promotes diabetic corneal wound healing through molecular mechanisms mediated via the adenosine 2B receptor Pubmed:29662125
Family Practice and Palliative Care Relationship of serum netrin-1 levels with breast masses Doi: 10.22391/fppc.467700
JOURNAL OF BIOLOGICAL CHEMISTRY Role of microRNA in CB1 antagonist-mediated regulation of adipose tissue macrophage polarization and chemotaxis during diet-induced obesity Pubmed: 30910812
International Journal of Molecular Sciences Downregulation of the Netrin-1 Receptor UNC5b Underlies Increased Placental Angiogenesis in Human Gestational Diabetes Mellitus Pubmed: 30897795
Cancer Biomark Serum netrin-1 as a biomarker for colorectal cancer detection Pubmed: 32474463
Níveis Elevados de Netrina-1 e IL-1β em Mulheres Idosas com SCA: Pior Prognóstico no Acompanhamento de Dois Anos
Tuberk Toraks Association of plasma netrin-1 level with inflammatory balance and comorbidities in patients with acute exacerbation of COPD 33853303
Adv Healthc Mater Conductive Hydrogel Conduits with Growth Factor Gradients for Peripheral Nerve Repair in Diabetics with Non‐suture Tape Pubmed:35670309
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB827Mu01 Recombinant Netrin 1 (Ntn1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB827Mu02 Recombinant Netrin 1 (Ntn1) Positive Control; Immunogen; SDS-PAGE; WB.
APB827Mu01 Active Netrin 1 (Ntn1) Cell culture; Activity Assays.
PAB827Mu02 Polyclonal Antibody to Netrin 1 (Ntn1) WB; IHC; ICC; IP.
PAB827Mu01 Polyclonal Antibody to Netrin 1 (Ntn1) WB; IHC; ICC; IP.
LAB827Mu81 FITC-Linked Polyclonal Antibody to Netrin 1 (Ntn1) WB; IHC; ICC; IF.
LAB827Mu71 Biotin-Linked Polyclonal Antibody to Netrin 1 (Ntn1) WB; IHC; ICC.
SEB827Mu ELISA Kit for Netrin 1 (Ntn1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB827Mu Multiplex Assay Kit for Netrin 1 (Ntn1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.