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Active Carboxylesterase 1 (CES1)

ACAT; CEH; CES2; HMSE1; PCE1; SES1; TGH; Retinyl ester hydrolase; Serine esterase 1; Triacylglycerol hydrolase; Egasyn; Cocaine carboxylesterase; Liver Carboxylesterase 1

ACTIVITY TEST

carboxylesterase 1 (CES1) also known as Liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant human CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50 mM Tris, pH 7.5( Assay Buffer), initiated by addition 50 μL of various concentrations of CES1 (dilute by Assay Buffer) to 50 µL of 2 mM Substrate 4-NPA(100 mM stock in Acetone, dilute by deionized water). Incubated at 37℃ for 10min, then read at a wavelength of 400 nm.
One unit of enzyme activity is defined as the 1μg of enzyme required to convert 1pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37°C. The specific activity of recombinant human CES1 is 600 pmol/min/µg.
Specific Activity (pmol/min/µg)=
△OD=Adjusted for Substrate Blank
F=Conversion Factor(convert from standard curve of 4-Nitrophenol)
T= Time
N=Amount of enzyme

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
International Journal of Nanomedicine Gold Nanoparticles Perturb Drug-Metabolizing Enzymes and Antioxidants in the Livers of Male Rats: Potential Impact on Drug Interactions Pubmed: 32764932
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
EPC374Hu61 Eukaryotic Carboxylesterase 1 (CES1) Positive Control; Immunogen; SDS-PAGE; WB.
APC374Hu01 Active Carboxylesterase 1 (CES1) Cell culture; Activity Assays.
RPC374Hu01 Recombinant Carboxylesterase 1 (CES1) Positive Control; Immunogen; SDS-PAGE; WB.
APC374Hu61 Active Carboxylesterase 1 (CES1) Cell culture; Activity Assays.
PAC374Hu01 Polyclonal Antibody to Carboxylesterase 1 (CES1) WB; IHC; ICC; IP.
SEC374Hu ELISA Kit for Carboxylesterase 1 (CES1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC374Hu Multiplex Assay Kit for Carboxylesterase 1 (CES1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.