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Active Endothelial Cell Specific Molecule 1 (ESM1)

Endocan

ACTIVITY TEST

Endothelial cell-specific molecule 1 (ESM1) is a proteoglycan secreted by endothelial cells (primarily in the human lung and kidney tissues) and its mRNA expression is regulated by inflammatory cytokines. Endocan expression, which detected in various epithelia and adipocytes has been shown to be upregulated by vascular endothelial growth factor (VEGF), fibroblast growth factor 2 (FGF-2), TNF alpha, IL1 beta, or lipopolysaccharide and downregulated by IFN gamma. Genetically engineered cells overexpressing ESM1 induce tumor formation, implying that ESM1 might be involved in the pathophysiology of tumor growth in vivo. Besides, Lymphocyte Function Associated Antigen 1 Alpha (LFA1a) has been identified as an interactor of ESM1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ESM1 and recombinant human LFA1a. Briefly, ESM1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to LFA1a-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti- ESM1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of ESM1 and LFA1a was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Urology Detection on dynamic changes of endothelial cell specific molecule-1 in acute rejection after renal transplantation PubMed: 22608803
Microvasc Res. Endocan is useful biomarker of survival and severity in sepsis. Pubmed: 24769132
J Matern Fetal Neonatal Med. Endocan, a putative endothelial cell marker, is elevated in preeclampsia, decreased in acute pyelonephritis, and unchanged in other obstetrical syndromes Pubmed:25211383
Microvasc Res. Endocan is useful biomarker of survival and severity in sepsis Pubmed:24769132
Angiology Endocan Levels and Subclinical Atherosclerosis in Patients With Systemic Lupus Erythematosus PubMed: 26614790
Archives of Medical Science - Atherosclerotic Diseases Assessment of subclinical cardiac damage in chronic plaque psoriasis patients: a case control study Journal:-102
Int Immunopharmacol.  Inhibition of endocan attenuates monocrotaline-induced connective tissue disease related pulmonary arterial hypertension. pubmed:27912147
Oncotarget Diagnostic and prognostic values of endothelial-cell-specific molecule-1 with malignant pleural effusions in patients with non-small cell lung cancer pubmed:28514746
Biomedical and Pharmacology Journal Changes in Endocan Levels and Blood Coagulation in HIV Infection DOI: 10.13005/bpj/1082
Life Sciences A novel role of endocan in alleviating LPS-induced acute lung injury Pubmed:29627442
International Urology and Nephrology Is the plasma endocan level a reliable predictor for the severity of erectile dysfunction? Pubmed:30054863
The Open Rheumatology Journal Serum Level of Endothelial Cell-Specific Molecule-1 (ESM-1) as a New Potential Biomarker for Rheumatoid Arthritis Disease Activity Doi: 10.2174/1874312901812010189
Molecular Medicine Reports Downregulation of ENDOCAN in myeloid leukemia cells inhibits proliferation and promotes apoptosis by suppressing nuclear factor‑κB activity Pubmed: 30816462
Biomarkers in Medicine Endothelial biomarkers in the light of new sepsis definition Pubmed: 30920847
Experimental and Therapeutic Medicine Risk stratification and prognostic evaluation of endothelial cell‑specific molecule1, von Willebrand factor, and a disintegrin‑like and metalloprotease with …
Turkish Journal of Medical Sciences A novel marker relationship between carotid intima–media thickness and disease activity score-28 in patients with rheumatoid arthritis: human endothelial cell … Pubmed: 31655533
Biomarkers Role of Ascitic Endocan Levels in the Diagnosis of Spontaneous Bacterial Peritonitis in Decompensated Cirrhosis Pubmed: 32364003
J Obstet Gynaecol Res Comparison of the maternal serum endocan levels in preterm premature rupture of membrane and normal pregnancy 34109715
turkish journal clinics laboratory The relationship between serum endocan levels with the presence of contrast-induced nephropathy in patients undergoing coronary angiography
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APC463Hu01 Active Endothelial Cell Specific Molecule 1 (ESM1) Cell culture; Activity Assays.
RPC463Hu01 Recombinant Endothelial Cell Specific Molecule 1 (ESM1) Positive Control; Immunogen; SDS-PAGE; WB.
PAC463Hu01 Polyclonal Antibody to Endothelial Cell Specific Molecule 1 (ESM1) WB; IHC; ICC; IP.
LAC463Hu71 Biotin-Linked Polyclonal Antibody to Endothelial Cell Specific Molecule 1 (ESM1) WB; IHC; ICC.
MAC463Hu22 Monoclonal Antibody to Endothelial Cell Specific Molecule 1 (ESM1) WB
MAC463Hu25 Monoclonal Antibody to Endothelial Cell Specific Molecule 1 (ESM1) WB
SEC463Hu ELISA Kit for Endothelial Cell Specific Molecule 1 (ESM1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC463Hu Multiplex Assay Kit for Endothelial Cell Specific Molecule 1 (ESM1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.