Active Histidine Rich Glycoprotein (HRG)

HPRG; HRGP; Histidine-Proline Rich Glycoprotein; Thrombophilia Due To Elevated HRG

ACTIVITY TEST

Human histidine-rich glycoprotein (HRG) is a multidomain, monomeric, secreted, 67-75 kDa member of the cystatin superfamily of molecules. Its name derives from the fact that 26% of its amino acids (aa) are histidine and proline. In human, it is synthesized as a 525 aa precursor that contains an 18 aa signal sequence and a 507 aa mature region. Five distinct domains are recognized in the mature molecule. As HRG has the function of cell adhesion, we measure the activity of recombinant human HRG by the ability of the immobilized protein to support the adhesion of con-A activated MOLT4 human acute lymphoblastic leukemia cells. When 1 x 105 cells/well with 7.5 ug/ml conA are added to recombinat human HRG coated plates (0.1 µg/mL with 100 µL/well), >70% cells will adhere after 2 hour incubation at 37 ℃. The adhesion of MOLT-4 after 2 hour incubation at 37 ℃ observed by inverted microscope was shown in Figure 1.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
J. Proteome Res. Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC–MS/MS ACS: pr300330z
Medical Oncology Evaluation of histidine-rich glycoprotein tissue RNA and serum protein as novel markers for breast cancer Springer: Source
Int J Cancer The nuclear bile acid receptor FXR controls the liver derived tumor suppressor histidine‐rich glycoprotein PubMed: 25363753
Nucleic Acid Therapeutics Pharmacokinetic and Pharmacodynamic Investigations of ION-353382, a Model Antisense Oligonucleotide: Using Alpha-2-Macroglobulin and Murinoglobulin Double-Knockout Mice Pubmed:27031383
United States Patent Application Compositions and Methods for Diagnosing Lung Cancer y2016:0077095.html
Hypertension Research Combination of serum histidine-rich glycoprotein and uterine artery Doppler to predict preeclampsia Pubmed:29416108
journal of histochemistry & cytochemistry Ultrastructural Localization of Histidine-rich Glycoprotein in Skeletal Muscle Fibers: Colocalization With AMP Deaminase Pubmed: 31880188
DOKLADY BIOCHEMISTRY AND BIOPHYSICS Personalized Approach to Determination of Histidine-Rich Glycoprotein and E-Cadherin in Supernatants of Immunocompetent Blood Cells and Breast Biopsy … Pubmed: 32342301
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC534Hu01 Recombinant Histidine Rich Glycoprotein (HRG) Positive Control; Immunogen; SDS-PAGE; WB.
APC534Hu61 Active Histidine Rich Glycoprotein (HRG) Cell culture; Activity Assays.
EPC534Hu61 Eukaryotic Histidine Rich Glycoprotein (HRG) Positive Control; Immunogen; SDS-PAGE; WB.
PAC534Hu01 Polyclonal Antibody to Histidine Rich Glycoprotein (HRG) WB; IHC; ICC; IP.
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MAC534Hu24 Monoclonal Antibody to Histidine Rich Glycoprotein (HRG) WB; IHC; ICC; IP.
MAC534Hu22 Monoclonal Antibody to Histidine Rich Glycoprotein (HRG) WB; IHC; ICC; IP.
MAC534Hu21 Monoclonal Antibody to Histidine Rich Glycoprotein (HRG) WB; IHC; ICC; IP.
MAC534Hu23 Monoclonal Antibody to Histidine Rich Glycoprotein (HRG) WB; IHC; ICC; IP.
MAC534Hu26 Monoclonal Antibody to Histidine Rich Glycoprotein (HRG) WB; IHC; ICC; IP.
SEC534Hu ELISA Kit for Histidine Rich Glycoprotein (HRG) Enzyme-linked immunosorbent assay for Antigen Detection.
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