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Active R-Spondin 1 (RSPO1)

RSPONDIN; Roof plate-specific spondin-1

ACTIVITY TEST

R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares~40% amino acid (aa) identity with three other R-Spondin family members. R-Spondin 1 competes with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization. Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate. Besides, Leucine Rich Repeat Containing G Protein Coupled Receptor 5 (LGR5) has been identified as an interactor of RSPO1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human RSPO1 and recombinant mouse LGR5. Briefly, biotin-linked RSPO1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to LGR5-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450nm immediately. The binding activity of recombinant human RSPO1 and recombinant mouse LGR5 was shown in Figure 1, the EC50 for this effect is 0.099 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Scand J Rheumatol.? The association of radiographic progression with serum R-spondin 1 (RSPO1) levels or Dickkopf-1 (DKK1)/RSPO1 ratios in rheumatoid arthritis patients: clinical evidence for reciprocal inhibition between DKK1 and RSPO1. Pubmed:25178409
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPM171Hu02 Recombinant R-Spondin 1 (RSPO1) Positive Control; Immunogen; SDS-PAGE; WB.
APM171Hu61 Active R-Spondin 1 (RSPO1) Cell culture; Activity Assays.
EPM171Hu61 Eukaryotic R-Spondin 1 (RSPO1) Positive Control; Immunogen; SDS-PAGE; WB.
RPM171Hu01 Recombinant R-Spondin 1 (RSPO1) Positive Control; Immunogen; SDS-PAGE; WB.
PAM171Hu01 Polyclonal Antibody to R-Spondin 1 (RSPO1) WB; IHC; ICC; IP.
MAM171Hu21 Monoclonal Antibody to R-Spondin 1 (RSPO1) WB; IHC; ICC; IP.
SEM171Hu ELISA Kit for R-Spondin 1 (RSPO1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMM171Hu Multiplex Assay Kit for R-Spondin 1 (RSPO1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.