ELISA Kit for Visfatin (VF)
Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase
- Product No.CEA638Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range1.23-100ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.51ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 665
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This assay has high sensitivity and excellent specificity for detection of Visfatin (VF).
No significant cross-reactivity or interference between Visfatin (VF) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Visfatin (VF) and the recovery rates were calculated by comparing the measured value to the expected amount of Visfatin (VF) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Visfatin (VF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Visfatin (VF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Visfatin (VF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Brazilian Journal of Biomoricity||EFFECT OF 8 WEEKS ENDURANCE TRAINING ON PLASMA VISFATIN IN MIDDLE-AGED MEN Brjb: source|
|Diabetes Research and Clinical Practice||The relationship between visfatin and HOMA-IR in hypertensive patients, and the effect of antihypertensive drugs on visfatin and HOMA-IR in hypertensive patients with insulin resistance ScienceDirect: S0168822711002956|
|J Physiol Pharmacol.||Early-effect of bariatric surgery (Scopinaro method) on intestinal hormones and adipokines in insulin resistant Wistar rat. Pubmed: 24304571|
|Endocr J.||CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes Pubmed:25168658|
|Exp Ther Med.||Plasma visfatin levels and mRNA expression of visfatin in peripheral blood mononuclear cells and peripheral blood monocyte-derived macrophages from normal weight females with polycystic ovary syndrome Pubmed:Pmc3991522|
|Eur J Cancer Prev.||Visfatin derived from ascites promotes ovarian cancer cell migration through Rho/ROCK signaling-mediated actin polymerization. Pubmed:25055182|
|Genome||Visfatin −948G/T and resistin −420C/Gpolymorphisms in Egyptian type 2 diabetic patients with and without cardiovascular diseases Nrcresearchpress:Source|
|PLOS ONE||Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting Drug PubMed: 26262991|
|Alcoholism and Drug Addiction||Changes in concentration of visfatin during four weeks of inpatient treatment of alcohol dependent males Science: Article|
|Cytokine||Variation in blood levels of hormones in obese patients following weight reduction induced by endoscopic and surgical bariatric therapies PubMed: 26539806|
|Genome||Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 2 Diabetic Patients With and Without Complications PubMed: 25120107|
|Acta Histochemica||Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines science:S0065128116301234|
|Endocrine, Metabolic & Immune Disorders-Drug Targets||Changes in Serum Nampt Levels and Its Significances in Diabetic Nephropathy Patients-The Potential Role of Nampt in T2DM with Diabetic Nephropathy. pubmed:28699485|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA638Hu01||Recombinant Visfatin (VF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|CPA638Hu21||OVA Conjugated Visfatin (VF)||Immunogen; SDS-PAGE; WB.|
|PAA638Hu01||Polyclonal Antibody to Visfatin (VF)||WB; IHC; ICC; IP.|
|PAA638Hu08||Polyclonal Antibody to Visfatin (VF)||WB; IHC; ICC; IP.|
|MAA638Hu22||Monoclonal Antibody to Visfatin (VF)||WB; IHC; ICC; IP.|
|SEA638Hu||ELISA Kit for Visfatin (VF)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CEA638Hu||ELISA Kit for Visfatin (VF)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA638Hu11||ELISA Kit DIY Materials for Visfatin (VF)||Main materials for "Do It (ELISA Kit) Yourself".|