ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)
P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide
- Product No.CEA957Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range2.47-200ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.91ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 630
For more details, please contact local distributors! US$ 2835
For more details, please contact local distributors! US$ 5355
For more details, please contact local distributors! US$ 44100
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Procollagen I N-Terminal Propeptide (PINP).
No significant cross-reactivity or interference between Procollagen I N-Terminal Propeptide (PINP) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Procollagen I N-Terminal Propeptide (PINP) and the recovery rates were calculated by comparing the measured value to the expected amount of Procollagen I N-Terminal Propeptide (PINP) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procollagen I N-Terminal Propeptide (PINP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procollagen I N-Terminal Propeptide (PINP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Procollagen I N-Terminal Propeptide (PINP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Trials||Is there benefit in optimising heart failure treatment in over-80 year-old patients? (HF-80 study): study protocol for a randomized controlled trial. Trials: 1745-6215|
|Clinical Biochemistry||Measurement of aminoterminal propeptide of type I procollagen (PINP) in serum ScienceDirect: S0009912012001543|
|Therapeutic Apheresis and Dialysis||Low molecular weight iron dextran increases fibroblast growth factor-23 concentration, together with parathyroid hormone decrease in hemodialyzed patients PubMed: 22458393|
|International Journal of Clinical & Experimental Pathology||IL4 and IL-17A provide a Th2/Th17-polarized inflammatory milieu in favor of TGF-β1 to induce bronchial epithelial-mesenchymal transition (EMT) PubMed: PMC3726963|
|Osteoporosis International||PINP as a biological response marker during teriparatide treatment for osteoporosis Pubmed: 24599274|
|Clinical Cancer Research||Zoledronic acid has differential anti-tumour activity in the pre-and post-menopausal bone microenvironment in vivo Aacrjournals: Source|
|Endocrinology||Oxytocin Reverses Ovariectomy-Induced Osteopenia and Body Fat Gain Endocrine: Source|
|general endocrinology||Oxytocin reverse ovariectomy-induced Osteoper and body fat gain Pubmed:24506069|
|J Neurol Sci.||The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism. Pubmed:24629477|
|Bone.||Neurofibromin inactivation impairs osteocyte development in Nf1Prx1 and Nf1Col1 mouse models Pubmed:24947449|
|Journal of Materials Science: Materials in Medicine||Phosphatidylethanolamine biomimetic coating increases mesenchymal stem cell osteoblastogenesis Pubmed:24980874|
|Springerplus.||Effects of de-escalated bisphosphonate therapy on bone turnover biomarkers in breast cancer patients with bone metastases Pubmed:25332877|
|Pharmacology||Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy PubMed: 25871861|
|frontiers in endocrinology||Oxytocin reverses osteoporosis in a sex-dependent manner PubMed: 26042090|
|MERIT RESEARCH JOURNALS||Changes in Bone Metabolism Months after Laparoscopic Morbidly Obese Pre-menopausal Women Mms: Content|
|Heart Rhythm.||Prognostic value of collagen turnover biomarkers in cardiac resynchronization therapy: A subanalysis of the TRUST CRT randomized trial population Pubmed:26776557|
|BMJ Open Sport Exerc Med||Procollagen markers in microdialysate can predict patient outcome after Achilles tendon rupture content:2|
|Bibliothekssystem Universität Hamburg||Bilaterale Kommunikation zwischen Osteoblasten und Osteoklasten volltexte:2016|
|Inflammation Research||Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy pubmed:27516211|
|Endocrine||Bone turnover response is linked to both acute and established metabolic changes in ultra-marathon runners pubmed:27422791|
|Heart & Vessels||Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR pubmed:28004175|
|journal of science and medicine in sport||The effect of calcium and vitamin D supplementation on bone health of male Jockeys. pubmed:27568072|
|Combined aerobic and resistance training improves bone health of female cancer survivors S2352187216300389|
|Cellular Physiology and Biochemistry||Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy pubmed:27889753|
|Epilepsy Research||The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats pubmed:27838501|
|Sci Rep.||Sprint Interval Training Induces A Sexual Dimorphism but does not Improve Peak Bone Mass in Young and Healthy Mice. pubmed:28303909|
|Metabolism.||Low periostin levels in adult patients with Langerhans cell histiocytosis are independently associated with the disease activity. pubmed:28521873|
|Heart and vessels||Erratum to: Effects on bone metabolism markers and arterial stiffness by switching to rivaroxaban from warfarin in patients with atrial fibrillation pubmed:28314975|
|SCIENCE & TECHNOLOGY||A Study Protocol: Spinal Morphology, Physical Performance, Quality of Life and Biochemical Markers in Adults at Risk of Osteoporotic Fractures 59f7c85aaca272607e2d8d7a|
|Knee Surg Sports Traumatol Arthrosc||Achilles tendon rupture healing is enhanced by intermittent pneumatic compression upregulating collagen type I synthesis pubmed:28668970|
|Method for the treatment of cardiovascular fibrosis patents:US9594083|
|Clinical Cases in Mineral and Bone Metabolism||Periostin and sclerostin levels in juvenile Paget's disease pubmed:29263750|
|Journal of Clinical Research in Pediatric Endocrinology||Congenital Hypothyroidism and Bone Remodeling Cycle pubmed:27840329|
|Biomarkers Medicial||Relationship of biomarkers of extracellular matrix with myocardial function in Type 2 diabetes mellitus pubmed:28685602|
|Heart and Vessels||Left ventricular reverse remodeling is not related to biopsy‑detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR 10.1007/s00380-016-0930-y|
|BioMed Research International||Optimization of the Time Window of Interest in Ovariectomized Imprinting Control Region Mice for Antiosteoporosis Research pubmed:29119115|
|Cytokine||12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis. pubmed:28460256|
|Advanced Healthcare Materials||Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events. pubmed:29120858|
|Bone.||The key role of proinflammatory cytokines, matrix proteins, RANKL/OPG and Wnt/β-catenin in bone healing of hip arthroplasty patients pubmed:29129760|
|Cell Death And Differentiation||TSC1 regulates osteoclast podosome organization and bone resorption through mTORC1 and Rac1/Cdc42 Pubmed:29358671|
|Nutrients||Specific Collagen Peptides Improve Bone Mineral Density and Bone Markers in Postmenopausal Women—A Randomized Controlled Study Pubmed:29337906|
|Osteoporosis International||Two-year cortical and trabecular bone loss in CKD-5D: biochemical and clinical predictors Pubmed:28993865|
|Journal of Nutrition||Cholecalciferol Supplementation Promotes Bone Turnover in Chinese Adults with Vitamin D Deficiency Pubmed:29897564|
|European Journal of Pharmacology||The effect of levetiracetam on rat bone mineral density, bone structure and biochemical markers of bone metabolism Pubmed:29428468|
|Journal of Clinical Densitometry||Geometric and mechanical bone response to a multidisciplinary weight loss intervention in adolescents with obesity: The ADIBOX study Pubmed:30076009|
|Hernia||Circulating matrix metalloproteinases and procollagen propeptides in inguinal hernia Pubmed:29484522|
|Pharmacology||Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats Pubmed:29898457|