ELISA Kit for Albumin (ALB)
- Promotion products
- Product No.CEB028Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range246.9-20,000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 89.6ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Albumin (ALB).
No significant cross-reactivity or interference between Albumin (ALB) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Albumin (ALB) and the recovery rates were calculated by comparing the measured value to the expected amount of Albumin (ALB) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Albumin (ALB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Albumin (ALB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Albumin (ALB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|PLoS One||Simvastatin Reduces Endotoxin-Induced Acute Lung Injury by Decreasing Neutrophil Recruitment and Radical Formation PubMed: 22701728|
|Liver International||Albumin administration protects against bilirubin-induced auditory brainstem dysfunction in Gunn rat pups Onlinelibrary: liv.12219|
|Journal of Infectious Diseases||Adjunctive corticosteroid therapy improves lung immunopathology and survival during severe secondary pneumococcal pneumonia in mice Pubmed: 24273183|
|Toxins (Basel).||Increased Circulating Levels of Vitamin D Binding Protein in MS Patients Pubmed:25590278|
|Mol Cell Biochem||The wnt/β-catenin signaling pathway participates in rhein ameliorating kidney injury in DN mice PubMed: 26346164|
|PLoS One||Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human Study Pubmed:26934615|
|Journal of Biomaterials and Tissue Engineering||3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering art00007|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|NPB028Hu01||Native Albumin (ALB)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPB028Hu01||Recombinant Albumin (ALB)||Positive Control; Immunogen; SDS-PAGE; WB.|
|EPB028Hu51||Eukaryotic Albumin (ALB)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB028Hu07||Polyclonal Antibody to Albumin (ALB)||WB; IHC; ICC; IP.|
|PAB028Hu05||Polyclonal Antibody to Albumin (ALB)||WB; IHC; ICC; IP.|
|LAB028Hu71||Biotin-Linked Polyclonal Antibody to Albumin (ALB)||WB; IHC; ICC.|
|LAB028Hu81||FITC-Linked Polyclonal Antibody to Albumin (ALB)||WB; IHC; ICC; IF.|
|MAB028Hu22||Monoclonal Antibody to Albumin (ALB)||WB; IHC; ICC; IP.|
|HEB028Hu||High Sensitive ELISA Kit for Albumin (ALB)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CEB028Hu||ELISA Kit for Albumin (ALB)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|AEB028Hu||ELISA Kit for Anti-Albumin Antibody (Anti-ALB)||Enzyme-linked immunosorbent assay for Antibody Detection.|
|ACB028Hu||CLIA Kit for Anti-Albumin Antibody (Anti-ALB)||Chemiluminescent immunoassay for Antibody Detection.|
|CCB028Hu||CLIA Kit for Albumin (ALB)||Chemiluminescent immunoassay for Antigen Detection.|
|KSB028Hu11||ELISA Kit DIY Materials for Albumin (ALB)||Main materials for "Do It (ELISA Kit) Yourself".|