ELISA Kit for Albumin (ALB)
- Product No.CEB028Rb
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range617.3-50,000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 304.9ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 406
For more details, please contact local distributors! US$ 1827
For more details, please contact local distributors! US$ 3451
For more details, please contact local distributors! US$ 28420
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Albumin (ALB).
No significant cross-reactivity or interference between Albumin (ALB) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Albumin (ALB) and the recovery rates were calculated by comparing the measured value to the expected amount of Albumin (ALB) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Albumin (ALB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Albumin (ALB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Albumin (ALB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|PLoS One||Simvastatin Reduces Endotoxin-Induced Acute Lung Injury by Decreasing Neutrophil Recruitment and Radical Formation PubMed: 22701728|
|Liver International||Albumin administration protects against bilirubin-induced auditory brainstem dysfunction in Gunn rat pups Onlinelibrary: liv.12219|
|Journal of Infectious Diseases||Adjunctive corticosteroid therapy improves lung immunopathology and survival during severe secondary pneumococcal pneumonia in mice Pubmed: 24273183|
|Toxins (Basel).||Increased Circulating Levels of Vitamin D Binding Protein in MS Patients Pubmed:25590278|
|Mol Cell Biochem||The wnt/β-catenin signaling pathway participates in rhein ameliorating kidney injury in DN mice PubMed: 26346164|
|PLoS One||Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human Study Pubmed:26934615|
|Journal of Biomaterials and Tissue Engineering||3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering art00007|
|Experimental and molecular pathology||Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. pubmed:29246788|
|Inflammation||Resolvin D1 Promotes SIRT1 Expression to Counteract the Activation of STAT3 and NF-κB in Mice with Septic-Associated Lung Injury Pubmed:30014231|
|Journal of Developmental Origins of Health and Disease||Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspring Pubmed:29582727|
|Medicine||Electret filter collects more exhaled albumin than glass condenser: A method comparison based on human study Pubmed:29384875|
|Stem Cell Research & Therapy||Therapeutic efficiency of human amniotic epithelial stem cell-derived functional hepatocyte-like cells in mice with acute hepatic failure Pubmed: 30463600|
|JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A||Decellularized Human Liver Scaffold‐Based Three‐dimensional Culture System Facilitate HBV Infection Pubmed: 30963688|
|biomedicine & pharmacotherapy||Treatment with 3, 4-dihydroxyphenylethyl alcohol glycoside ameliorates sepsis-induced ALI in mice by reducing inflammation and regulating M1 polarization Pubmed: 31146107|
|biochemical and biophysical research communications||Anti-proteinuria effect of antibody against ANGPTL3 coil-coiled domain on adriamycin-induced nephropathy in mice Pubmed: 31256934|
|Diabetes, Metabolic Syndrome and Obesity||A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activation Pubmed: 31447572|
|Catalog No.||Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species||Applications (RESEARCH USE ONLY!)|
|NPB028Rb01||Native Albumin (ALB)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPB028Rb01||Recombinant Albumin (ALB)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPB028Rb02||Recombinant Albumin (ALB)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB028Rb52||Polyclonal Antibody to Albumin (ALB)||WB; IHC; ICC; IP.|
|PAB028Rb51||Polyclonal Antibody to Albumin (ALB)||WB; IHC; ICC; IP.|
|LAB028Rb81||FITC-Linked Polyclonal Antibody to Albumin (ALB)||WB; IHC; ICC; IF.|
|LAB028Rb71||Biotin-Linked Polyclonal Antibody to Albumin (ALB)||WB; IHC; ICC.|
|CEB028Rb||ELISA Kit for Albumin (ALB)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSB028Rb11||ELISA Kit DIY Materials for Albumin (ALB)||Main materials for "Do It (ELISA Kit) Yourself".|